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Gabor Patonay Professor, Analytical Chemistry BIS Environmental Advisor Ph.D., Technical University of Budapest, Hungary, (1979) Dr. Gabor Patonay Department of Chemistry Georgia State University P.O. Box 4098 Atlanta, Georgia 30302-4098
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Our studies are centered on analytical chemistry applications with special emphasis on bioanalytical analyses. Two major analytical techniques are used: fluorescence spectroscopy and capillary electrophoresis (CE). Our fluorescence spectroscopy research efforts are focused on the longer wavelength region of the electromagnetic spectrum, the near-infrared (NIR) region. The NIR region is advantageous due to the inherent low interference and the high molar absorptivities of the NIR absorbing chromophores. When NIR spectroscopy is coupled with the use of semiconductor lasers and detectors, an especially useful technique emerges. Semiconductor lasers have all the properties of other types of lasers with the added benefits of compactness, lower power consumption and low cost.
We are currently studying several bioanalytical applications. These include, but are not limited to, NIR DNA sequencing; NIR fluorescence immunoassays, including fiber optic and conventional applications; DNA probe techniques; pH, metal ion and hydrophobicity determinations. Additionally, new environmentally important NIR analytical techniques are studied in our research group, including separation and bioremediation applications. Another component of our studies is the development of new biosensors using fiber optic techniques. Our CE research is mainly focused on new high sensitivity detection methods, bioseparations and NIR labeling techniques.
The latest area of our research is development of new spectroscopic method for forensic applications. Our research group developed a new latent blood detection method using new fluorescin chemistry. In addition to latent blood detection we also develop new analytical techniques for latent fingerprint detection and determination of other chemical residues, e.g., pepper spray residues, gun shot residues and explosive residues. The most promising part of our research is the combination of forensic analytical chemistry with NIR spectroscopy.
Representative Publications:
Testino, Samuel A.; Patonay, Gabor, High-throughput inhibition screening of major human cytochrome P450 enzymes using an in vitro cocktail and liquid chromatography-tandem mass spectrometry, Journal of Pharmaceutical and Biomedical Analysis (2003), 30(5), 1459-1467.
L. Strekowski, Mason, C.L , Lee, H,. R. Gupta, J. Sowell, and Gabor Patonay, Syntesis of Water Soluble Near-Infrared Cyanine Dyes Functionalized with [(Succinimido)oxy]carbonyl Group, J. Hetercyc. Chem, (2003) 40, 913-916,.
Ellis,A.L., Mason,J.C., Lee,H.W., Strekowski,L., Patonay,G., Choi,H., and Yang,J.J. (2002). Design, synthesis, and characterization of a calcium-sensitive near infrared dye. Talanta, 56, 1099-1107.
Tarazi,L., Choi,H., Mason,J.C., Sowell,J., Strekowski,L., and Patonay,G. (2002) Characterization of a novel crown ether-bearing near-infrared heptamethine cyanine dye. A study of fluorescence quenching by lithium. Microchemical Journal, 72, 55-62.
Tarazi,L., Narayanan,N., Sowell,J., Patonay,G., and Strekowski,L. (2002). Investigation of the spectral properties of a squarylium near-infrared dye and its complexation with Fe(III) and Co(II) ions. Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 58, 257-264.
Strekowski,L., Gorecki,T., Mason,J.C., Lee,H., and Patonay,G. (2001). New heptamethine cyanine reagents for labeling of biomolecules with a near-infrared chromophore. Heterocyclic Communications, 7, 117-122.
Sowell,J., Agnew-Heard,K.A., Mason,J.C., Mama,C., Strekowski,L., and Patonay,G. (2001). Use of non-covalent labeling in illustrating ligand binding to human serum albumin via affinity capillary electrophoresis with near-infrared laser induced fluorescence detection. Journal of Chromatography B, 755, 91-99.
Strekowski,L., Mason,J.C., Britton,J.E., Lee,H., Van Aken,K., and Patonay,G. (2000). The addition reaction of hydroxide or ethoxide ion with benzindolium heptamethine cyanine dyes. Dyes and Pigments, 46 163-168.
Tarazi,L., Narayanan,N., and Patonay,G. (2000). Investigation of the spectral properties of a squarylium near-infrared dye and its complexation with Fe(III) and Co(II) ions. Microchemical Journal, 64, 247-256.
Meadows,F., Narayanan,N., and Patonay,G. (2000). Determination of protein-dye association by near infrared fluorescence-detected circular dichroism. Talanta, 50, 1149-1155.
Daneshvar, M.I., Peralta, J.M., Casay, G.A., Narayanan, N., Evans, L., Patonay, G., & Strekowski, L. (1999). Detection of Biomolecules in the Near-Infrared Spectral Region via a Fiber-Optic Immunosensor. Journal Of Immunological Methods 226, 119-128.
Evans, L., & Patonay, G. (1999). Effects Of Organic and Aqueous Solvents on the Electronic Absorption and Fluorescence of Chloroaluminum (III) Tetrasulphonated Naphthalocyanine. Talanta 48, 933-942.
Baars, M.J., & Patonay, G. (1999). Ultrasensitive Detection of Closely Related Angiotensin I Peptides doing Capillary Electrophoresis with Near-Infrared Laser-Induced Fluorescence Detection. Analytical Chemistry 71, 667-671.
Baars, M.J., & Patonay, G. (1999). Interfacing a High-Sensitivity, Near-Infrared Laser-Induced Fluorescence Detector with a Commercial Capillary Electrophoresis Instrument. Applied Spectroscopy 52, 1619-1622.
Patonay, G., George, A. & Echols, P. (1998). Why to use AOTF in NIR Dye Spectroscopy. Abstracts Of Papers Of The American Chemical Society 215, U88-U89
Tarazi, L., George, A., Patonay, G. & Strekowski, L. (1998). Spectral Characterization of a Novel Near-Infrared Cyanine Dye: a Study of Its Complexation with Metal Ions. Talanta 46, 1413-1424.
George, A. & Patonay, G. (1997). Fluorescence Studies of Carbocyanines Using AOTF. Talanta 45, 285-289.
Hofstraat, J.W., vanHouwelingen, G.D.B., Schotman, A.H.M., Nuijens, M.J., Gooijer, C., Velthorst, N.H., Strekowski, L. & Patonay, G. (1997). Near-IR Fluorescent Labels for Determination of Functional Groups in Polymeric Materials. Polymer 38, 4033-4041.
Mason, J.C., Patonay, G. & Strekowski, L. (1997). A New pH-Sensitive Near-Infrared Chromophore. Heterocyclic Communications 3, 409-411.
Sophianopoulos, A.J., Lipowski, J., Narayanan, N. & Patonay, G. (1997). Association of Near-Infrared Dyes with Bovine Serum Albumin. Applied Spectroscopy 51, 1511-1515.
Swamy, A.R., Danesvar, M.I., Evans, L., Strekowski, L., Narayanan, N., Szurdoki, F., Wengatz, I., Hammock, B.D. & Patonay, G. (1997). Near-Infrared Fluorescent Immunoassays: A Novel Approach to Environmental Analysis. Acs Symposium Series 657, 146-161.
Williams, R.J., Peralta, J.M., Tsang, V.C.W., Narayanan, N., Casay, G.A., Lipowska, M., Strekowski, L. & Patonay, G. (1997). Near-Infrared Heptamethine Cyanine Dyes: A New Tracer for Solid-Phase Immunoassays. Applied Spectroscopy 51, 836-843.
Williams, R.J., Peralta, J.M., Tsang, V.C.W., Narayanan, N., Casay, G.A., Lipowska, M., Strekowski, L. & Patonay, G. (1997). Near Infrared Heptamethine Cyanine Dyes: A New Tracer for Solid-Phase Immunoassays (vol 51, pg 836, 1997). Applied Spectroscopy 51, 1435-1435.
Casay, G.A., Narayanan, N., Evans, L., Czuppon, T. & Patonay, G. (1996). Near-Infrared tetra-Substituted Aluminum 2,3-Naphthalocyanine Dyes for Optical Fiber Applications. Talanta 43, 1997-2005.
Gorecki, T., Patonay, G., Strekowski, L., Chin, R. & Salazar, N. (1996). Synthesis of Novel Near-Infrared Cyanine Dyes for Metal Ion Determination. Journal Of Heterocyclic Chemistry 33, 1871-1876.
Patonay, G., Daneshvar, M., Swamy, A. & Strekowski, L. (1996). NIR Fluorescent Immunoassays and their Application for Environmental Measurements. Abstracts Of Papers Of The American Chemical Society 211, 24.
Strekowski, L., Lipowska, M., Gorecki, T., Mason, J.C. & Patonay, G. (1996). Functionalization of Near-Infrared Cyanine Dyes. Journal Of Heterocyclic Chemistry 33, 1685-1688.
Casay, G.A., Meadows, F., Daniels, N., Roberson, A. & Patonay, G. (1995). Fiber Optic Probe Applications Using Near-Infrared Compounds - Determination of NaOH. Spectroscopy Letters 28, 301-326.
Kuklenyik, P. & Patonay, G. (1995). Simple HPLC Detector for the Detection of NIR Dyes. Instrumentation Science & Technology 23, 113-122.
Narayanan, N., Strekowski, L., Lipowska, M. & Patonay, G. (1997). A New Method for the Synthesis of Heptamethine Cyanine Dyes: Synthesis of New Near Infrared Fluorescent Labels. (1995). J.Org.Chem. 62, 9387-9387.
"Spectral Characterization and Evaluation of Modified Near-Infrared Laser-Dyes for DNA-Sequencing", Shealy, D. B.; Lohrmann, R.; Ruth, J. R.; Narayanan, N.; Sutter, S. L.; Casay, G. A.; Evans, L.; Patonay, G. Appl. Spectrosc., 1995, 49 1815.
"Direct Determination of Substituted Azepinoindole Enantiomers in Rat Plasma Using Silica Stationary-Phase and beta-Cyclodextrin as a Mobile-Phase Additive", Pullen, R. H.; Brennan, J. J.; Lammers, R.; Patonay, G. Anal. Chem. 1995 67, 1903.
"Fiber Optic Probe Applications Using Near-Infrared Compounds - Determination of NaOH", Casay, G. A.; Meadows, F.; Daniels, N.; Roberson, A.; Patonay, G. Spectrosc. Lett. 1995, 28, 301.
"Simple HPLC Detector for the Detection of NIR Dyes", Kuklenyik, P.; Patonay, G. Instrumen. Sci. & Techn. 1995, 23 113.
"Chiral Separation Retention Mechanisms in High-Performance Liquid-Chromatography Using Bare Silica Stationary-Phase and beta-Cyclodextrin as a Mobile-Phase Additive", Pullen, R. H.; Brennan, J. J.; Patonay, G. J. Chrom. part A 1995, 691, 187.