SELECTED RECENT PUBLICATIONS:
Jozef Salon, Jia Sheng, Jianhuan Gan, Zhen Huang*, "Synthesis and crystal structure of 2’-Se-modified guanosine containing DNA", 2009, J. Org. Chem., minor revision is in progress.
Jia Sheng and Zhen Huang*, "Selenium Derivatization of Nucleic Acids for X-ray Crystal Structure and Function Studies", (invited and peer reviewed), Chemistry and Biodiversity (John Wiley & Sons, Inc.), accepted, 2009.
Jia Sheng, Abdalla Hassan, Zhen Huang*, "Synthesis of the First Tellurium-Derivatized Oligonucleotides for Structural and Functional Studies", Chemistry - A European Journal, 2009, 15, 10210-10216.
Abdalla E. A. Hassan, Jia Sheng, Jiansheng Jiang, Wen Zhang, and Zhen Huang*, “Synthesis and Crystallographic Analysis of 5-Se-Thymidine DNAs”, Organic Letter, 2009, 11, 2503-2506. (This paper is highlighted as a featured article by Chemical & Engineering News, vol. 87, no. 23, p. 51 on June 8, 2009.)
Lina Lin, Jia Sheng, Razin K. Momin, Quan Du, Zhen Huang*, “Facile synthesis and anti-tumor cell activity of Se-containing nucleosides”, Nucleosides, Nucleotides and Nucleic Acids, 2009, 28, 56-66.
Julianne Caton-Williams, Zhen Huang*, "Synthesis and DNA Polymerase Incorporation of Colored 4-Selenothymidine Triphosphate with a Single Atom Substitution", Angewandte Chemie Int. Ed., 2008, 47, 1723-1725.
Jia Sheng, Abodalla E.A. Hassan and Zhen Huang*, “Telluride-Mediated Elimination and Novel Synthesis of 2’,3’-Didehyro-2’,3’-dideoxynucleosides (d4Ns)”, Journal of Organic Chemistry, 2008, 73, 3725-3729.
Jozef Salon, Jiansheng Jiang, Jia Sheng, Oksana O. Gerlits, Zhen Huang*, "Derivatization of DNAs with Selenium at 6-Position of Guanine for Function and Crystal Structure Studies", Nucleic Acids Research, 2008, 36, 7009-7018.
Jia Sheng and Zhen Huang*, "Selenium Derivatization of Nucleic Acids for Phase Determination in Nucleic Acid X-ray Crystallography"
(invited and peer reviewed), International Journal of Molecular Sciences, 2008, 9, 258-271.Julianne Caton-Williams and Zhen Huang*, "Biochemistry of Naturally-occurring and Unnatural Selenium-derivatized Nucleic Acids" (invited and peer reviewed), Chemistry and Biodiversity (John Wiley & Sons, Inc.), 2008
, 5, 396-407.Jia Sheng and Zhen Huang*, "Synthesis of a 4-Se-Thymidine Phosphoramidite and Its Incorporation into Oligonucleotides for Structure and Function Studies" (invited and peer reviewed), Current Protocols in Nucleic Acid Chemistry (John Wiley & Sons, Inc.), 2008,
Chapter 1, Unit 1.19.Minyong Li, Na Lin, Zhen Huang, Lupei Du, Craig Altier, Hao Fang, and Binghe Wang*, “Selecting aptamers for a glycoprotein through the incorporation of the boronic acid moiety”, Journal of American Chemical Society, 2008, 130, 12636-12638.
Yuchun Tang, Yan-Qing Zhang, Zhen Huang, Xiaohua Hu, Yichuan Zhao, "Recursive Fuzzy Granulation for Gene Subsets Extraction and Cancer Classification," (peer reviewed journal), IEEE Transactions on Information Technology in Biomedicine, 2008, 12, 723-730.
Jozef Salon, Jia Sheng, Jiansheng Jiang, Guexiong Chen, Julianne Caton-Williams, Zhen Huang*, “Oxygen Replacement with Selenium at the Thymidine 4-position for the Se-Base-Paring and Crystal Structure Studies”, Journal of American Chemical Society, 2007, 129, 4862-4863.
Jiansheng Jiang, Jia Sheng, Nicolas Carrasco, and Zhen Huang*, “Selenium Derivatization of Nucleic Acids for Crystallography”, Nucleic Acids Research, 2007, 35, 477-485.
Jia Sheng, Jiansheng Jiang, Jozef Salon, and Zhen Huang*, “Synthesis of a 2’-Se-Thymidine Phosphoramidite and Its Incorporation into Oligonucleotides for Crystal Structure Study”, Organic Letters, 2007, 9, 749-752.
Na Lin, Jun Yan, Zhen Huang, Craig Altier, Minyong Li, Nicolas Carrasco, Mitsu Suyemoto, Lynette Johnston, Siming Wang, Qian Wang, Hao Fang, Julianne Caton-Williams, Binghe Wang*, “Design and synthesis of boronic acid-labeled thymidine triphosphate for incorporation into DNA”, Nucleic Acids Research, 2007, 35: 1222 -1229.
Yuchun Tang, Yan-Qing Zhang, Zhen Huang, “Development of Two-Stage SVM-RFE Gene Selection Strategy for Microarray Expression Data Analysis” (peer reviewed journal), IEEE/ACM Transactions on Computational Biology and Bioinformatics (TCBB), 2007, 4, 365-381.
Nicolas Carrasco, Julianne Canton-Williams, Gary Brandt, Siming Wang, and Zhen Huang*, “Efficient Enzymatic Synthesis of Phosphoroselenoate RNA Using Adenosine 5’-(
a-P-seleno)triphosphate”, Angewandte Chemie Int. Ed., 2006, 45, 94-97.Gary Brandt, Nicolas Carrasco, and Zhen Huang*, “RNA Substrate Cleavage Catalyzed by Phosphoroselenoate Hammerhead Ribozymes Transcribed Using 5’-(α-P-Seleno)Triphosphates”, Biochemistry, 2006, 45, 8972-8977.
Gianina Logan,# Charity Igunbor# (# equal contribution), Gue-Xiong Chen, Hays Davis, Arlyne Simon, Jozef Salon, Zhen Huang*, “A Novel and Simple Strategy for Incorporation, Protection, and Deprotection of Selenium Functionality”, Synlett, 2006, 10, 1554-1558.
Jozef Salon, Guexiong Chen, Yoani Portilla, Markus W. Germann, and Zhen Huang*, “Synthesis of a Novel 2’-Se-uridine Phosphoramidite and Its Incorporation into Oligonucleotides for Structure Study”, Organic Letters, 2005, 7, 5645-5648.
Yuchun Tang, Yan-Qing Zhang, and Zhen Huang, “FCM-SVM-RFE Gene Feature Selection Algorithm for Leukemia Classification from Microarray Gene Expression Data” (peer reviewed), Proceedings of Fuzzy Systems (FUZZ-IEEE), 2005, 97-101.
Yuchun Tang, Yan-Qing Zhang, Zhen Huang, and Xiaohua Hu, "Granular SVM-RFE Gene Selection Algorithm for Reliable Prostate Cancer Classification on Microarray Expression Data" (peer reviewed), Proceedings of Bioinformatics & Bioengineering (BIBE), 2005, 290-293.
Yuchun Tang, Yuanchen He, Yan-Qing Zhang, Zhen Huang, Xiaohua Tony Hu, and Rajshekhar Sunderraman, “A Hybrid CI-Based Knowledge Discovery System on Microarray Gene Expression Data” (peer reviewed), Proceedings of Computational Intelligence in Bioinformatics and Computational Biology (IEEE CIBCB), 2005, 25-30.
Mohammed Alsaidi, Elena Lum, and Zhen Huang*, “Direct Detection of a Specific Cellular mRNA on Functionalized Microplate”, ChemBioChem, 2004, 5, 1136-1139.
Yuri Buzin, Nicolas Carrasco, and Zhen Huang*, “Synthesis of Selenium-Derivatized Cytidine and Oligonucleotides for X-ray Crystallography Using MAD”, Organic Letters, 2004, 6, 1099-1102.
Nicolas Carrasco, Yuri Buzin, Elizabeth Tyson, Elizer Halpert, and Zhen Huang*, “Selenium derivatization and crystallization of DNA and RNA oligonucleotides for X-ray crystallography using multiple anomalous dispersion”, Nucleic Acids Research, 2004, 32, 1638-1646.
Nicolas Carrasco and Zhen Huang*, “Enzymatic Synthesis of Phosphoroselenoate DNA using Thymidine 5’-(a-P-Seleno)-triphosphate and DNA Polymerase for X-ray Crystallography using MAD”, Journal of American Chemical Society, 2004, 126, 448-449.
Zhen Huang,* and Mohammed Alsaidi, “Selective Labeling and Detection of Specific mRNA in a Total RNA Sample”, Analytical Biochemistry, 2003,
322, 269-274.Zhen Huang*, and Jack W. Szostak, “Selective Labeling of RNA in an RNA Mixture”, Analytical Biochemistry, 2003, 315, 129-133.
Zhen Huang,* and Jack W. Szostak, “Evolution of Aptamers with a New Specificity and New Secondary Structures from an ATP Aptamer”, RNA, 2003, 9, 1456-1463.
Quan Du, Nicolas Carrasco, Marlanna Teplova, Christopher J. Wilds,
Martin Egli, and Zhen Huang*, “Internal Derivatization of
Oligonucleotides with Selenium for X-ray Crystallography Using MAD”,
Journal of American Chemical
Society.
Zhen Huang and Steven A. Benner,* "Oligodeoxyribonucleotide Analogs with Bridging Dimethylenesulfide, Sulfoxide and Sulfone Groups: Towards a Second Generation Model of Nucleic Acid Structure", Journal of Organic Chemistry, 2002,
67, 3996-4013.
Nicolas Carrasco, Dov
Ginsburg, Quan Du, and Zhen Huang*, “Synthesis of Selenium-Derivatized Nucleosides
and Oligonucleotides for X-ray Crystallography”,
Nucleosides, Nucleotides, & Nucleic Acids, 2001,
vol. 20, issue 9,
1723-1734.
Zhen Huang* and Jun Zhao, “In vitro Selection of Ligand-binding and Catalytic RNA and DNA”, Frontiers of Biotechnology and Pharmaceuticals, 2001, Vol. 2, Science Press Ltd., New York, pp9-31.
Zhen Huang, Ming Guo, John Reiner, Kang Zhao, Frontiers of Biotechnology and Pharmaceuticals, Vol. 2, Science Press Ltd., New York, (June, 2001).
PATENT and APPLICATIONS:
Zhen Huang and Sarah M. Spencer, “RNA Microchip for Rapid, Direct and Specific Detection of RNAs”, 2009, US provisional patent application, submitted.
Zhen Huang, “RNA detection and quantitation”, 2008, US patent, 7,354,716.
Zhen Huang and Jia Sheng, Title: “Telluride-Mediated Elimination and Novel Synthesis of 2’,3’-Didehyro-2’,3’-dideoxynucleosides (d4Ns)”, 2008, 61/038,439.
Binghe Wang, Mingyong Li, Zhen Huang, and Shilong Zheng, Title: "Nucleotides containing boronic acid groups, polynucleotides containing boronic acid groups, aptamers containing boronic acid groups, and methods or preparation of each", 2007, US Provisional Patent Application, pending (60/950,681).
Zhen Huang, Title: “Incorporation of 4-Se-Thymidine into Oligonucleotides”, 2006, US Provisional Patent Application, pending (60/871,598).
Zhen Huang, Title: “RNA Selective Detection and Quantitation using RNA Chip and Plate”, 2006, Europe Patent Application, pending (04780049.5-2402).
Zhen Huang, Title: “RNA Selective Detection and Quantitation using RNA Chip and Plate”, 2006, Australia Patent Application (pending).
Zhen Huang, Title: “RNA Selective Detection and Quantitation using RNA Chip and Plate”, 2004, US Utility Patent Application, 10/912,556.
Zhen Huang, Title: “Synthesis of Selenium-Derivatized Nucleosides, Nucleotides, Phosphoramidites, Triphosphates and Nucleic Acids”, 2002, US Patent Application, 10/104,995. Both Glen Research, Inc. and Dharmacon Research, Inc. licensed this invention.
Zhen Huang, Title: “Vacuum-Operated Tube Washer and Dryer”, 2002, US Patent Application, 10,233,379.
Zhen Huang and Jack W. Szostak, Title: "A Simple and Efficient Method to Label and Modify 3’-Termini of RNA Using DNA Polymerase and a Synthetic Template with Defined Overhung Nucleotides", 2001, US Patent, US 6,238,865.