Curriculum Vitae-Chuan He

 

Address

 

CIS 319B, 929 East 57th Street

Department of Chemistry

The University of Chicago

Chicago, IL 60637

USA

Tel: 773-702-5061. Fax: 773-702-0805. Email: chuanhe@uchicago.edu

 

Education

 

·       University of Science and Technology of China, Bachelor of Science in Chemistry, June, 1994.

·       Massachusetts Institute of Technology, Ph.D. in Chemistry, August, 2000.

·       Harvard University, Postdoc in Chemical Biology, 2000-2002. 

·        

Employment

 

·       Massachusetts Institute of Technology, Teaching Assistant, 1995-1996.

·       Massachusetts Institute of Technology, Research Assistant, 1996-1997.

·       Massachusetts Institute of Technology, Merck/MIT Graduate Fellow, 1997-1999.

·       Massachusetts Institute of Technology, Research Assistant, 1999-2000.

·       Harvard University, Damon Runyon Cancer Foundation Postdoctoral Fellow, 2000-2002.

·       The University of Chicago, Assistant Professor, 2002-present

 

Awards

 

·       Merck/MIT Graduate Fellowship, MIT, 1997-1999.

·       The fourth annual Davison Prize for the best thesis in Inorganic Chemistry, MIT, 2001.

·       Postdoctoral Fellowship from the Damon Runyon Cancer Research Foundation, 2000-2002.

·       Searle Scholar Award, 2003

·       Research Corporation Research Innovation Award, 2003

·       G&P Foundation for Cancer Research Young Investigation Award, 2004

·       W. M. Keck Foundation Distinguished Young Scholar in Medical Research, 2004

·       A.P. Sloan Fellow, 2005

·       Arnold and Mabel Beckman Foundation Young Investigator, 2005      

·       Research Corporation Cottrell Scholar, 2005

·       National Science Foundation CAREER Award, 2005

·       Camille Dreyfus Teacher-Scholar Award, 2006

 

Other Professional Activities

 

·       Ad hoc reviewers for National Institutes of Health, National Science Foundation, and Department of Energy Office of Basic Energy Sciences

Selected peer-reviewed publications (in chronological order).

 

1.         He, C. and Lippard, S. J. “Aminoguanidinium Hydrolysis Effected by a Hydroxo-Bridged Dicobalt(II) Complexes”, J. Am. Chem. Soc. 1998, 120, 105-113.

2.         He, C. and Lippard, S. J. “Modeling Carboxylate-Bridged Dinuclear Active Sites in Metalloenzymes Using a Novel Naphthyridine-Based Dinucleating Ligand”, J. Am. Chem. Soc. 2000, 122, 184-185.

3.         Kaminskaia, N. V.; He, C. and Lippard, S. J. “Reactivity of -Hydroxodizinc(II) Centers in Enzymatic Catalysis through Model Studies”, Inorg. Chem. 2000, 39, 3365-3373.

4.         He, C.; Victoria, G.; Spingler, B. and Lippard, S. J. “Monodentate-Bridged Phosphodiester and Sulfate Complexes: Structural Insight into the Biological Activation of Phosphodiesters, Sulfate, and Sulfate Esters”, Inorg. Chem. 2000, 39, 4188-4189.

5.         He, C. and Lippard, S. J. “Design and Synthesis of Multidentate Dinucleating Ligands Based on 1,8-Naphthyridine”, Tetrahedron 2000, 56, 8245-8252.

6.         He, C. and Lippard, S. J. “Synthesis and Characterization of Several Dicopper(I) Complexes and a Spin-Delocalized Dicopper(I,II) Mixed-Valence Complex Using a 1,8-Naphthyridine-Based Dinucleating Ligand”, Inorg. Chem. 2000, 39, 5225-5231.

7.         He, C.; Barrios, A. M.; Lee, D.; Kuzelka, J.; Davydov, R. M. and Lippard, S. J. “Diiron Complexes of 1,8-Naphthyridine-Based Dinucleating Ligands as Models for Hemerythrin”, J. Am. Chem. Soc. 2000, 122, 12683-12690.

8.         He, C. and Lippard, S. J. “Synthesis and Electrochemical Studies of Diiron Complexes of 1,8-Naphthyridine-Based Dinucleating Ligands to Model Features of the Active Sites of Non-Heme Diiron Enzymes”, Inorg. Chem. 2001, 40, 1414-1420 (cover story).

9.         He, C.; DuBois, J. L.; Hedman, B.; Hodgson, K. O. and Lippard, S. J. “A short Copper-Copper Distance in a (-1,2-Peroxo)dicopper(II) Complex Having 1,8-Naphthyridine Unit as an Additional Bridge”, Angew. Chem. Int. Ed. Engl. 2001, 40, 1484-1487.

10.        He, C. and Verdine, G. L. “Trapping Distinct Structural States of a Protein/DNA Interaction through Disulfide Cross-linking”, Chem. Biol. 2002, 9, 1297-1303.

11.        He, C.; Wei, H. and Verdine, G. L. “Converting the Sacrificial DNA Repair Protein N-Ada into a Catalytic Methyl Phosphotriester Repair Enzyme”, J. Am. Chem. Soc. 2003, 125, 1450-1451.

12.        Mishina, Y. and He, C. “Probing the Structure and Function of E. coli DNA Alkylation Repair AlkB Protein through Chemical Crosslinking”, J. Am. Chem. Soc.  2003, 125, 8730-8731.

13.        Duguid, E. M.; Mishina, Y. and He, C. “How Do DNA Repair Proteins Locate Base Lesions? A Chemical Crosslinking Method to Investigate O6-Alkylguanine-DNA Alkyltransferases”, Chem. Biol.  2003, 10, 827-835.

14.       Cui, Y. and He, C. “Efficient Aziridination of Olefins Catalyzed by A Unique Disilver(I) Compound”, J. Am. Chem. Soc. 2003, 125, 16202-16203.

15.       Chen, P. and He, C. “Constructing Highly Sensitive and Selective Fluorescent Biosensors for Metal Ions by Using the MerR Family Proteins”, J. Am. Chem. Soc. 2004, 126, 728-729.

16.       He, C. and Mishina, Y. “Modeling Non-Heme Iron Proteins”, Curr. Opin. Chem. Biol. 2004, 8, 201-208.

17.       Mishina, Y.; Lee, C.-H. J. and He, C. “Potential DNA Preferences of Human and Bacterial AlkB Proteins as Revealed from Chemical Crosslink Studies”, Nucleic Acids Res. 2004, 32, 1548-1554.

18.       Shi, Z. and He, C. “An Au-Catalyzed Cyclialkylation of Electron Rich Arenes with Epoxides to Prepare 3-Chromanols”, J. Am. Chem. Soc. 2004, 126, 5964-5965.

19.       Shi, Z. and He, C. “Efficient Functionalization of Aromatic C–H Bonds Catalyzed by Gold(III) under Mild and Solvent Free Conditions”, J. Org. Chem. 2004, 69, 3669-3671.

20.       Cui, Y. and He, C. “A Silver-Catalyzed Intramolecular Amidation of Saturated C–H bonds”, Angew. Chem. Int. Ed.  2004, 43, 4210-4212.

21.       Shi, Z. and He, C. “Direct Functionalization of Arenes by Primary Alcohol Sulfonate Esters Catalyzed by Gold(III)”, J. Am. Chem. Soc. 2004, 126, 13596-13597.

22.       Mishina, Y.; Chen, L. X. and He, C. “Preparation and Characterization of the Native Iron(II)-Containing AlkB Protein Directly from Escherichia coli”, J. Am. Chem. Soc. 2004, 126, 16930-16936.

23.       Chen, P.; Greenberg, B.; Taghavi, S.; Romano, C.; van der Lelie, D. and He, C. “A Novel Lead(II) Regulatory Protein in Ralstonia Metallidurans: Development of A Ratiometric Fluorescent Lead(II) Sensor”, Angew. Chem. Int. Ed. 2005, 44, 2715-2719.

 24.       Yang, C.-G. and He, C. “Gold(I)-Catalyzed Intermolecular Addition of Phenols and

            Carboxylic Acids to Olefins”, J. Am. Chem. Soc. 2005, 127, 6966-6967.

25.       Duguid, E. M.; Rice, P. A. and He, C. “Implications for How the Human DNA Repair Protein AGT Locates Damage as Revealed from A Unique X-ray Structure of Its DNA Bound Form”, J. Mol. Biol. 2005, 350, 657-666.

26.             He, C.; Hus, J.-C.; Sun, L. J.; Zhou, P.; Norman, D. P. G.; Dötsch, V.; Wei, H.; Gross, J.

D.; Lane, W. S.; Wagner, G. and Verdine, G. L. “A Methylation-Dependent Electrostatic Switch Controls DNA Repair and Transcriptional Activation by E. coli Ada”, Mol. Cell. 2005, 20, 117-129.

27.       Li, Z.; Shi, Z. and He, C. “Addition of Heterocycles to Electron Deficient Olefins and Alkynes Catalyzed by Gold(III)”, J. Organomet. Chem. 2005, 690, 5049-5054 (an invited contribution).

 28.       Mishina, Y.; Yang, C.-G. and He, C. “Direct Repair of the Exocyclic DNA Adduct 1,N6-

            Ethenoadenine by the AlkB Proteins”, J. Am. Chem. Soc. 2005, 127, 14594-14595.

29.       Yang, C.-G.; Reich, N. W. and He, C. “Intramolecular Additions of Alcohols and Carboxylic Acids to Inert Olefins Catalyzed by Silver(I) Triflate”, Org. Lett. 2005, 7, 4553-4556.

30.       Mishina, Y.; Duguid, M. E. and He, C. “Direct Repair of DNA Alkylation Damage”, Chem. Rev. 2006, 106, 215-232.

31.       Zhang, J.; Yang, C.-G. and He, C. “Gold(I)-Catalyzed Intra- and Intermolecular Hydroamination of Unactivated Olefins”, J. Am. Chem. Soc. 2006, 128, 1798-1799.

32.       Brouwer, C. and He, C. “Efficient Gold-Catalyzed Hydroamination of 1,3-Dienes” Angew. Chem. Int. Ed. 2006, 45, 1744-1747.

33.       Reich, N. W.; Yang, C.-G.; Shi, Z. and He, C. “Gold(I)-Catalyzed Synthesis of Dihydrobenzofurans from Aryl Allyl Ethers” Synlett 2006, 8, 1278-1280 (an invited contribution).

34.       Mishina, Y. and He, C. “Oxidative Dealkylation DNA Repair Mediated by the Mononuclear Non-Heme Iron AlkB Proteins” J. Inorg. Biochem. 2006, 100, 670-678 (an invited review).

35.       Li, Z. and He, C. “Recent Advances in Silver-Catalyzed Nitrene, Carbene, and Silylene-Transfer Reactions", Euro. J. Org. Chem. 2006, 4313-4322 (an invited review).

36.       Li, Z.; Ding, X. and He, C. “Nitrene Transfer Reactions Catalyzed by Gold Complexes” J. Org. Chem. 2006, 71, 5876-5880.

37.       Li, Z.; Zhang, J.; Brouwer, C.; Yang, C.-G.; Reich, N. W. and He, C. “Brønsted Acid Catalyzed Addition of Phenols, Carboxylic Acids and Tosylamides to Simple Olefin” Org. Lett. 2006, 8, 4175-4178.

38.       Chen, P.; Bae, T.; Williams, W. A.; Duguid, E. M.; Rice, P. A.; Schneewind, O. and He, C. “An Oxidation Sensing Mechanism is Used by A Global Regulator of Staphylococcus aureus” Nature Chem. Biol. 2006, 2, 591-595.

39.       Wegner, S. V.; Okesli, A.; Chen, P.; and He, C. “Design of An Emission Ratiometric Biosensor from MerR Family Proteins: A Sensitive and Selective Sensor for Hg2+” J. Am. Chem. Soc. 2007, 129, 3474-3475.

40.       Li, Z.; Capretto, D. A.; Rahaman, R. O. and He, C. “A Disilver-Catalyzed Intermolecular Amination of Saturated C–H Groups” Angew. Chem. Int. Ed. 2007, 46, 5184-5186.

41.       Li, Z.; Capretto, D. A. and He, C. “Gold(III)-Catalyzed Amination of Aromatic and Aliphatic C-H Groups” J. Am. Chem. Soc. Revision.

42.       Shan, G.; Li, Y.; Zhang, J.; Szulwach, K.; Li, Y.; Qin, Y.; Duan, R.; Faghihi, M. A.; Khalil, A.; Lu, L.; Chan, A.; Zhou, D.; Shi, Z.; Liu, Q.; Wahlestedt, C.; He, C. and Jin, P. “A Small Molecule Enhances RNA Interference and Promotes microRNA Processing” Science, pending final revision.

43.       Qiu, Z.; Lu, L. and He, C. “A Diazirine-Based Nucleoside Analogue for Efficient DNA Interstrand Photocross–Linking” J. Am. Chem. Soc. Revision.

44.       Chen, P. R.; Wasinger, E. C.; Zhao, J.; van derLelie, D.; Chen, L.  and He, C. “The Mechanism of the Selective Lead(II) Recognition by the PbrR691 Protein: Insights from the Spectroscopic Investigations” J. Am. Chem. Soc. Revision

45.       Chen, H; Wasinger, E. C.; Li, Z.; Brugarolas, P.; Chen, L. X. and He, C. “Pseudomonas putida CadR Recognizes Cadmium(II) at Least 200–Fold More Selectively Over Other Metal Ions” Submitted.

46.       Chen, J.; Karabkova, E.; Chen, H.; Jian, X.; He, C.* and Zhu, H.* “DNA Base Damage Recognition in E. Coli Proteome Using a Proteome Chip Approach” Submitted.

47.       Sarkar, S. K.; Andoy, N. M.; Benitez, J. J.; Chen, P. R.; Kong, J. S.; He, C. and Chen, P. “Engineered Holliday Junctions as Single-Molecule Reporters for Protein-DNA Interactions: Application to Metalloregulators” Submitted.

48.       Durek, T.; Zhang, J.; He, C. and Kent, S. B. H. “Synthesis of Photo-Active Analogs for a Cystine Knot Trypsin Inhibitor Protein” Submitted.

49.       Yang, C.-G.; Yi, C.; Duguid, E. M.; Sullivan, C. T.; Rice, P. A. and He, C. “Crystal Structures of the DNA/RNA Repair Enzymes AlkB and ABH2 Bound to DNA” Submitted.

 

Research Support

 

“DNA Base Repair and Modification: Novel Chemical Cross-Linking and Proteomic Approaches”.

Agency: W. M. Keck Foundation (2004 scholar)             07/01/2004-06/30/2009              

 

“A Chemical Proteomic Strategy to Study DNA Repair/Modification Proteins”

Agency: Arnold and Marble Beckman Foundation           06/01/2005 – 05/31/2008

 

“A Chemical Crosslinking Method to Study DNA Repair/Modification Proteins”

Agency: Research Corporation Cottrell Scholar Program 06/01/2005 – 05/31/2010           

 

“Chemistry and Catalysis of Silver and Gold”

Agency: A.P. Sloan Foundation Research Fellowship      09/16/2005-09/15/2007  

 

“Chemistry and Mechanism of Direct DNA Repair Proteins”                             

Agency: NIH/NIGMS 1 R01 GM071440-01                    08/01/2005 – 07/31/2010           

 

“Selective Recognition and Regulation of Lead(II) by the PbrR Proteins”

Agency: NSF CAREER award                                      01/01/2006 – 12/31/2010                                                                                   

“Developing Chemical Probes for Study DNA Repair and DNA Methylation/Demethylation”

Agency: Camille and Henry Dreyfus Foundation             06/01/2006 – 05/31/2011                                                           

“Selective Recognition of Heavy Elements by Protein-Based Reagents”              

Agency: DOE Office of Basic Energy Sciences              06/01/2007 – 05/31/2010

 

“Tuning Virulence Determinants in Staphylococcus aureus”                             

Agency: NIH/NIAID 1 R01 AI074658                           08/01/2007 – 07/31/2012                       

 

Presentations (after Aug. 2002)

 

Argonne National Laboratory, October 2002 “Silver-Catalyzed Organic Transformation”.

 

Miami University, Ohio, June 2003 “Damage-Searching and Repair Mechanisms of Alkylation DNA Repair Proteind”.

 

University of Chicago, Cancer Research Center, Section of Hematology/Oncology, September 2003 “Direct Repair of Alkylated DNA Damage”.

 

Illinois Institute of Technology, Chicago, IL, September 2003 “The Mechanism of the DNA Dealkylation Repair Proteins”.

 

2004 NSF Inorganic Workshop , Sedona AZ, June 10, 2004 “Chemistry and Catalysis of Silver and Gold Ions in Solution”.

 

American Chemical Society Anaheim National Meeting, March 28, 2004 “Study of Coinage Metal Ions”

 

The 8th International Symposium for Chinese Organic Chemists and the 5th International Symposium for Chinese Inorganic Chemists, Hong Kong, China, Dec. 21, 2004 “Chemical Methods to Study Protein-DNA Interactions”.

 

Brookhaven National Laboratory, Biology Department, Upton, NY, March 4, 2005 “Develop Metal Ion Biosensors and Study of DNA Repair”.

 

International symposium “New Generations and Perspectives of Organic Chemistry”, Kyoto University, Japan. Invited speaker on March 22, 2005 “Chemistry and Catalysis of Silver and Gold”.

 

Department of Synthetic Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan. March 23, 2005 “Chemical Biology of DNA Repair Proteins”.

 

Tokyo University, Japan. March 24, 2005 “Chemical Methods to Study Protein-DNA Interactions”.

 

Toray Industry Int. Kamakura, Japan. March 25, 2005 “Chemical Methods to Study Protein-DNA Interactions”.

 

2005 NSF Physical Organic/Macromolecule Workshop, Bodega Bay Lodge and Spa, CA, July 31-August 4, 2005 “Alkylation DNA Repair and Proteomics on DNA Repair Proteins”.

 

Department of Chemistry, UNC Chapel Hill, Sept. 13, 2005 “Chemical Methods to Study Protein/DNA Interactions”.

 

Department of Chemistry, The University of Chicago, Oct. 3, 2005 “Chemical Methods to Study Metalloregulation and DNA Repair”.

 

Department of Chemistry, University of Rochester, Oct. 26, 2005 “Chemical Methods to Study Protein-DNA Interactions”.

 

Department of Chemistry, Yale University, Dec. 6, 2005 “Silver and Gold Chemistry and Study of Metalloregulation”.

 

Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Dec. 7, 2005 “Chemical Methods to Study Metalloregulation and DNA Repair”.

 

Metals in Biology Gordon Research Conference, Jan. 30, 2006 “Regulation of Metals and Virulence/Drug Resistance in Bacteria”.

 

Department of Chemistry and Chemical Biology, Cornell University, Feb. 21, 2006 “Silver and Gold Chemistry and Study of Bacterial Regulation of Metals and Virulence/Drug Resistance”.

 

Department of Chemistry, UIUC, March 7, 2006 “Silver and Gold Chemistry and Study of Bacterial Regulation of Metals and Virulence/Drug Resistance”.

 

Searle Scholar Annual Meeting, April 11, 2006 “Regulation of Metals and Virulence/Drug Resistance in Bacteria”.

 

NSF Synthetic Organic Chemistry Workshop, June 2, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Virulence/Drug Resistance”.

 

2006 FASEB conference on Nucleic Acid Enzymes, June 12, 2006 “DNA Alkylation Repair”.

 

School of Chemistry and Material Science, USTC, P.R. China, July 4, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Metals, Virulence and Drug Resistance”.

 

The 1st Symposium on the Frontiers of Chemistry in the School of Chemistry and Chemical Engineering, Nanjing University, P.R. China, July 4, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Metals, Virulence and Drug Resistance”.

 

The Second Sino-US Chemistry Professor Conference, SIOC, P.R. China, July 8, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Virulence and Drug Resistance”.

 

College of Chemistry and Chemical Engineering, Beijing University, P.R. China, July 17, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Metals, Virulence and Drug Resistance”.

 

Department of Chemistry, Purdue University, August 29, 2006 “Silver Oxidation Chemistry and Bacterial Regulation of Metals, Virulence and Drug Resistance”.

 

Inorganic Research Conference of the European Science Foundation, Athens, Greece, Nov. 16, 2006 “Bacterial Regulation of Metals, Antibiotic Resistance and Virulence”.

 

The Woodward Lecture Series in Organic Chemistry, Department of Chemistry and Chemical Biology, Harvard University, Nov. 27, 2006 “Mechanisms of the Bacterial Regulation of Metals, Antibiotic Resistance and Virulence”.

 

Biochemistry Seminar, Department of Chemistry, Massachusetts Institute of Technology, Nov. 28, 2006 “Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Inorganic Chemistry Seminar, University of California-Berkeley, Dec. 1, 2006 “Silver Oxidation Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Inorganic Chemistry Seminar, Michigan State University, Dec. 4, 2006 “Silver and Gold Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Biochemistry Seminar, Department of BMBCB, Northwestern University, Jan. 8, 2007 “Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Biochemistry Seminar, Department of Biochemistry, Southwestern Medical Center, Feb. 15, 2007 “Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Inorganic Chemistry Seminar, Indiana University at Bloomington, March 2, 2007 “Silver and Gold Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Abbott Laboratory, March 8, 2007 “Silver and Gold Chemistry and Regulation of Virulence and Antibiotic Resistance in Pathogens”.

 

Department of Chemistry, University of Pennsylvania, March 13, 2007 “Silver Oxidation Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Department of Chemistry, Boston College, April 10, 2007 “Silver Oxidation Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Department of Chemistry, Brandeis University, April 11, 2007 “Silver and Gold Chemistry and Bacterial Regulation of Metal ions and Virulence/Antibiotic Resistance”.

 

Department of Chemistry, Illinois Institute of Technology, Student-invited seminar, April 13, “Silver Oxidation Chemistry and Regulation of Virulence, Antibiotic Resistance, and Metal Ions in Bacteria”.

 

Department of Chemistry, Northern Illinois University, April 16, “Silver Oxidation Chemistry and Regulation of Virulence and Antibiotic Resistance in Pathogens”.

 

W. M. Keck Foundation, April 24, “Study Protein-DNA Interactions”.

 

Chemistry Division, Argonne National Laboratory, May 15, “Silver and Gold Chemistry and
Bacterial Regulation of Metal Ions”.

 

Albany 2007: Conversation 15, Albany, New York, June 22, “Regulation of Virulence and Antibiotic Resistance in Pathogens”