Curriculum Vitae – Xi Chen
Department of Chemistry, University of California, Davis
One Shields Avenue, Davis, CA 95616, USA
Tel: 530-754-6037, Fax: 530-752-8995, E-mail: chen@chem.ucdavis.edu
Group page: http://chemgroups.ucdavis.edu/~chen/
B.S. in Chemistry: 1994, Xiamen University, Xiamen, Fujian, China.
Ph.D. in Chemistry: 2000, Wayne State University, Detroit, MI. Bio/Organic Chemistry
Dissertation title: a1,3-Galactosyltransferase and a-Gal epitope mediated cancer immunotherapy.
(Advisor: Dr. Peng G. Wang)
Postdoctoral Fellow: May-Dec., 2000, Wayne State University, Detroit, MI. (Advisor: Dr. Peng G. Wang)
· 8/03-present, Assistant Professor, University of California - Davis
Group: 5 postdoctoral researchers, 7 graduate students
Teaching: CHE 237, Bioorganic Chemistry (Fall 2003, Graduate course)
CHE118C, Organic Chemistry (Spring 2004, Undergraduate course)
CHE128C, Organic Chemistry (Fall 2004, Undergraduate course)
CHE129B, Organic Chemistry Laboratory (Winter 2005, Undergraduate lab course)
CHE150, Chemistry of Natural Products (Fall 2005, Undergraduate course)
CHE 118C, Organic Chemistry (Spring 2006, Undergraduate course)
CHE150, Chemistry of Natural Products (Fall 2006, Undergraduate course)
CHE128C, Organic Chemistry (Spring 2007, Undergraduate course)
Research Bioorganic Chemistry & Chemical Biology: Chemoenzymatic synthesis of oligosaccharides, polysaccharides, and glycoconjugates; X-ray crystal structure and mutagenesis studies of glycosyltransferases and other carbohydrate biosynthetic enzymes; functional studies of carbohydrates; site-specific glycoconjugation; carbohydrates in cancer
· 1/02-7/03, Scientist II, Neose Technologies, Inc.
Research Enzymatic remodeling of glycoproteins.
· 1/01-1/02, Scientist I, Neose Technologies, Inc.
Research Technology development for enzymatic synthesis of glycosaminoglycans (GAGs) with specific emphasis on heparin.
· 5/00 – 12/00, Postdoctoral Fellow, Department of Chemistry, Wayne State University.
Research Synthesis of carbohydrates through biosynthetic pathway engineering
· 1/98 – 5/00, Graduate Research Assistant, Department of Chemistry, Wayne State University.
Research Synthesis of carbohydrates through biosynthetic pathway engineering, genome-based chemistry, molecular cloning of glycosyltransferases and related enzymes, enzymology for glycosyltransferases and related enzymes, biocatalytic synthesis of oligosaccharides, carbohydrate based cancer immunology therapy.
· 8/97 - 1/98, Organic Chemistry Teaching Assistant, Department of Chemistry, Wayne State University.
· 8/96 - 8/97, Organic Laboratory Assistant, Department of Chemistry, University of Miami.
Research Kinetic analysis of protein tyrosine phosphatase, molecular cloning of galactosyltransferases
· 9/94 - 7/96, Organic Laboratory Teaching Assistant, Xiamen University, China.
Research Material chemistry, design, synthesis and applications of a series of carbohydrate-based polymers for oxygen-accumulating membranes.
· 9/90 - 7/94, Undergraduate Student, Xiamen University, China.
Research Material chemistry, study temperature controlled material for temperature sensitive heating plates.
09/2006-08/2009 Beckman Young Investigator Award
01/2006-12/2010 NSF CAREER Award
11/2000 Travel award for 5th Annual Conference of the Society for Glycobiology
07/1994 Excellent Graduate
07/1993 Jingxiong Fellowship
E. PROFESSIONAL SOCIETY MEMBERSHIPS:
2000-present American Society for Biochemistry and Molecular Biology
2000-present American Chemical Society
2000-present American Association for the Advancement of Science
2002-present Society for Glycobiology
2004-present Glycobiology Research and Training Center
F. PROFESSIONAL ACTIVITIES:
1. Reviewer for the following journals:
Biochemical and Biophysical Research Communications (1)
Biochemistry (2)
Bioorganic & Medicinal Chemistry (1)
Carbohydrate Chemistry (1)
Cancer Biotherapy & Radiopharmaceuticals (1)
Chemistry – A European Journal (2)
European Journal of Organic Chemistry (1)
Glycobiology (3)
Journal of Electroanalytical Chemistry (1)
Journal of Organic Chemistry (6)
Letters in Organic Chemistry (1)
Medicinal Research Reviews (2)
Organic Letters (8)
2. Proposal Reviewer
National Science Foundation (5 total, one in 2005, two in 2006, and two in 2007)
Ohio Cancer Research Associates Proposals, 2007 (1)
Research Corporation Cottrell College Science Award Proposal (1)
3. Reviewer for National Institutes of Health
A. July 14-15, 2005 Mail reviewer, National Cancer Institute, Applications for emerging technologies, special panel (Assigned 1 proposal)
B. March 16-17, 2006 National Cancer Institute, Applied Emerging Technologies for Cancer Research, Bethesda, MD 20814, special panel (Assigned 3 proposals)
C. June 14-15, 2006 NIH Study section SBCA, Ad hoc reviewer (Assigned 8 proposals)
4. Section Chair and Judge for poster section, XXIIIrd International Carbohydrate Symposium, Whistler, BC Canada (July 23-28, 2006)
5. One of the three organizers for Chemical Glycobiology Symposium in ACS San Francisco National Meeting (September 11-12, 2006)
6. Section Chair, The 9th International Symposium for Chinese Organic Chemists (ISCOC-9), Singapore (Dec. 17-21, 2006)
7. Consultant for Intelligent Optical Systems, Inc., Torrance, CA 90505 since Dec. 2005
8. One of the ACS symposium book series editors on Chemical Glycobiology. 2007
9. Core facilities evaluation for Jackson State University, July 9-12, 2007
G. PEER-REVIEWED PUBLICATIONS (IN CHRONOLOGICAL ORDER)
1. Fang, J.; Li, J.; Chen, X.; Zhang, Y.; Wang, J.; Guo, Z.; Zhang, W.; Yu, L.; Brew, K.; Wang, P. G. Highly efficient chemoenzymatic synthesis of a-galactosyl epitopes with a recombinant a(1,3)-galactosyltransferase. J. Am. Chem. Soc. 1998, 120, 6635-6638.
2. Chen, X.; Andreana, P. R.; Wang, P. G. Carbohydrates in transplantation. Curr. Opin. Chem. Biol. 1999, 3, 650-658.
3. Chen, X.; Kowal, P.; Hamad, S.; Fan, H.; Wang, P. G. Cloning, expression and characterization of a UDP-galactose 4-epimerase from E. coli. Biotech. Lett. 1999, 21, 1131-1135.
4. Wang, J.; Chen, X.; Zhang, W.; Zacharek, S.; Chen, Y.; Wang, P. G. Enhanced inhibition of human anti-Gal antibody binding to mammalian cells by synthetic a-Gal epitope polymers. J. Am. Chem. Soc. 1999, 121, 8174-8181.
5. Fang, J.; Chen, X.; Zhang, W.; Wang, J.; Andreana, P.; Wang, P. G. A unique chemoenzymatic synthesis of a-Gal epitope derivatives containing free amino groups: efficient separation and further manipulation. J. Org. Chem. 1999, 64, 4089-4094.
6. Li, J.; Zacharek, S.; Chen, X.; Wang, J.; Zhang, W.; Janczuk, A.; Wang, P. G. Bacteria targeted by human natural antibodies using a-Gal conjugated receptor-specific glycopolymers. Bioorgan. Med. Chem. 1999, 7, 1540-1558.
7. Janczuk, A.; Li, J.; Zhang, W.; Chen, X.; Chen, Y.; Fang, J.; Wang, J.; Wang, P. G. a-Gal oligosaccharides: chemistry and potential biomedical application. Curr. Med. Chem. 1999, 6, 155-164.
8. Zhang, W.; Xie, W.; Wang, J.; Chen, X.; Fang, J.; Chen, Y.; Li, J.; Yu, L.; Chen, D.; Wang, P. G. Recent progress in glycochemistry and green chemistry. Curr. Org. Chem. 1999, 3, 241-267.
9. Chen, X.; Liu, Z.; Wang, J.; Fang, J.; Fan, H.; Wang, P. G. Changing the donor cofactor of a1,3galactosyltransferase by fusion with UDP-Gal 4-epimerase: more efficient biocatalysis for synthesis of a-Gal epitopes. J. Biol. Chem. 2000, 275, 31594-31600.
10. Chen, X.; Zhang, W.; Wang J.; Fang, J.; Wang, P. G. Production of a-Gal epitopes by combined UDP-galactose 4-epimerase and a1,3-galactosyltransferase recombinant whole cells. Biotech. Prog. 2000, 16, 595-599.
11. Chen, X.; Kowal, P.; Wang, P. G. Large-scale biosynthesis of oligosaccharides. Curr. Opin. Drug Discovery & Development 2000, 3, 756-763.
12. Fang, J.; Chen, X.; Zhang, W.; Janczuk, A.; Wang, P. G. Synthesis of a-Gal epitope derivatives with a galactosyltransferase-epimerase fusion enzyme. Carbohydr. Res. 2000, 329, 873-878.
13. Xian, M.; Wang, K.; Chen, X.; Hou, Y.; McGill, A.; Chen, X.; Zhou, B.; Zhang, Z.; Cheng, J.; Wang, P. G. Inhibition of protein tyrosine phosphatases by low-molecular-weight S-nitrosothiols and S-nitrosylated human serum albumin. Biochem. Biophys. Res. Commun. 2000, 268, 310-314.
14. Chen, X.; Fang, J.; Zhang, J.; Liu, Z.; Shao, J.; Kowal, P.; Andreana, P.; Wang, P. G. Sugar nucleotide bead (superbead): a versatile tool for practical synthesis of oligosaccharides. J. Am. Chem. Soc. 2001, 123, 2081-2082.
15. Chen, X.; Zhang, J.; Kowal, P.; Liu, Z.; Andreana, P. R.; Lu, Y.; Wang, P. G. Transferring a biosynthetic cycle into a productive E. coli strain: large-scale synthesis of galactosides. J. Am. Chem. Soc. 2001, 123, 8866-8867.
16. Chen, Y.-S.; Zhang, W.; Chen, X.; Wang, J.-Q.; Wang, P. G. a-Gal-conjugated anti-rhinovirus agents: chemo-enzymatic syntheses and testing of anti-Gal binding. J. Chem. Soc., Perkin Trans. 1, 2001, 14, 1716-1722.
17. Kowal, P.; Chen, X.; Wang, P. G. Microbial glycosyltransferases. In: Glycochemistry: Principles, Synthesis and Applications. (Editors: Wang, P. G., Bertozzi, C. R.), Marcel Dekker, Inc., New York, NY. 2001, pp. 625-640.
18. Zhang, J.; Wu, B.; Liu, Z.; Kowal, P.; Chen, X.; Shao, J.; Wang, P. G. Large-scale synthesis of carbohydrates for pharmaceutical development. Curr. Org. Chem. 2001, 5, 1169-1176.
19. Chen, X.; Liu, Z.; Zhang, W.; Fang, J.; Andreana, P. R.; Wang, P. G. Large-scale synthesis of carbohydrate through biotechnology: Production of a-Gal epitopes by recombinant E. coli “superbug”. ChemBioChem. 2002, 3, 47-53.
20. Liu, Z.; Zhang, J.; Chen, X.; Wang, P. G. Combined biosynthetic pathway for de novo production of UDP-galactose: catalyzed with multiple enzymes immobilized on agarose beads. ChemBioChem 2002, 3, 348-355.
21. Chen, Y.; Janczuk, A.; Chen, X.; Wang, J.; Ksebati, M.; Wang, P. G. Expeditious syntheses of two carbohydrate-linked cisplatin analogs. Carbohydr. Res. 2002, 337, 1043-1046.
22. Nahalka, J.; Liu, Z.; Chen, X.; Wang, P. G. Superbeads: immobilization in “Sweet” chemistry. Chemistry 2003, 9, 372-377.
23. Chen, X.; Wu, B.; Wang, P. G. Glucuronides in anti-cancer therapy. Curr. Med. Chem.-Anti-Cancer Agents 2003, 3, 139-150.
24. Zhang, J.; Kowal, P.; Chen, X.; Wang, P. G. Large-scale synthesis of globotriose derivatives through recombinant E. coli. Org. Biomol. Chem. 2003, 1, 3048-3053.
26. Saribas, A. S.; Mobasseri, A.; Pristatsky, P.; Chen, X.; Barthelson, R.; Hakes, D.; Wang, J. Production of N-sulfated polysaccharides using yeast expressed N-deacetylase/N-sulfotransferase-1 (NDST-1). Glycobiology, 2004, 14, 1217-1228.
27. Yu, H.; Yu, H.; Karpel, R.; *Chen, X. Chemoenzymatic synthesis of CMP-sialic acid derivatives by a one-pot two-enzyme system: comparison of substrate flexibility of three microbial CMP-sialic acid synthetase. Bioorg. Med. Chem. 2004, 12, 6427-6435.
28. Yu, H.; Chokhawala, H.; Karpel, R.; Yu, H.; Wu, B.; Zhang, J.; Zhang, Y.; Jia, Q.; *Chen, X. A multifunctional Pasteurella multocida sialyltransferase: a powerful tool for the synthesis of sialoside libraries. J. Am. Chem. Soc. 2005, 127, 17618-17619.
29. Yu, H.; Ryan, W.; Yu, H.; *Chen, X. Characterization of a bifunctional cytidine 5’-monophosphate N-acetylneuraminic acid synthetase cloned from Streptococcus agalactiae. Biotechnol. Lett. 2006, 28, 107-113.
30. Ni, L.; Sun, M.; Yu, H.; Chokhawala, H.; *Chen, X.; *Fisher, A. J. Cytidine 5’-monophosphate (CMP)-induced structural changes in a multifunctional sialyltransferase from Pasteurella multocida. Biochemistry, 2006, 45, 2139-2148.
31. Yu, H.; Huang, S.; Chokhawala, H.; Sun, M.; Zheng, H.; *Chen X. Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural a-2,6-linked sialosides: a P. damsela a-2,6-sialyltransferase with extremely flexible donor substrate specificity. Angew. Chem. Int. Ed. 2006, 45, 3938-3944.
32. Yu, H.; *Chen X. Aldolase catalyzed synthesis of b-D-Gal-(1®9)-D-KDN: A novel acceptor for sialyltransferases. Org. Lett. 2006, 8, 2393-2396.
33. Yu, H.; Chokhawala, H.; Huang, S.; *Chen X. One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Nature Protocols. 2006, 1, 2485-2492.
34. Chokhawala, H.; *Chen X. Chemical aspects (scope and limitations) A: Enzymatic approaches to O-glycoside introduction: Glycosyltransferases. In Comprehensive Glycoscience, from Chemistry to Systems Biology (J. P. Kamerling, Ed.) Subject Editors: Geert-Jan Boons, YC Lee, Akemi Suzuki, Naoyuki Taniguchi, and Alphons G. J. Voragen. Elsevier, Oxford, UK, 2007. Invited review. In press.
35. Yu, H.; *Chen X. Enzymatic synthesis of carbohydrate-containing biomolecules. In Wiley Encyclopedia of Chemical Biology. John Wiley & Sons, Inc. NJ, 2007. Invited review. In press.
36. Chokhawala, H.; Yu, H.; *Chen X. High-throughput substrate specificity studies of sialidases using chemoenzymatically synthesized sialoside libraries. ChemBioChem 2007, 8, 194-201.
37. Huang, S.; Yu, H.; *Chen, X. Disaccharides as sialic acid aldolase substrates: synthesis of disaccharides containing a sialic acid at the reducing end. Angew. Chem. Int. Ed. 2007, 5, 2249-2253.
38. Yu, H.; *Chen, X. Carbohydrate post-glycosylational modifications. Org. Biomol. Chem. (Emerging Area Article) Featured as cover picture, 2007, 5, 865-872.
39. Ni, L.; Chokhawala, H. A.; Cao, H.; Henning, R.; Ng, L.; Huang, S.; Yu, H.; *Chen, X.; *Fisher, A. J. Crystal structures of a Pasteurella multocida sialyltransferase complexes with acceptor and donor analogs reveal substrate binding sites and catalytic mechanism. Biochemistry 2007, 46, 6288-6298.
40. Yu, H.; Chokhawala, H. A.; Varki, A.; *Chen, X. Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Org. Biomol. Chem. 2007, 5, 2458-2463.
41. Li, Y.; Sun, M.; Huang, S.; Yu, H.; Chokhawala, H. A.; Thon, V.; *Chen, X. The Hd0053 gene of Haemophilus ducreyi encodes an a2,3-sialyltransferase. Biochem. Biophys. Res. Commun. 2007, In press, doi:10.1016/j.bbrc.2007.07.063
42. Lewis, A. L.; Cao, H.; Patel, S. K.; Diaz, S.; Ryan, W.; Carlin, A. F.; Thon, V.; Lewis, W. G.; Varki, A.; Chen, X.; Nizet, V. NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharides in Group B Streptococcus. J. Biol. Chem. 2007, In press. doi:10.1074/jbc.M700340200
43. Chokhawala, H. A.; Cao, H.; Yu, H.; *Chen, X. Enzymatic synthesis of fluorinated mechanistic probes for sialidases and sialyltransferases. J. Am. Chem. Soc. 2007, In press.
44. Yu, H.; Chokhawala, H. A.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of sialosides and their applications. In Chemical Glycobiology ACS book series, Chen, X.; Halcomb, R.; Wang, P. G. Eds. In press.
45. Muthana, S.; Yu, H.; Huang, S.; *Chen, X. Chemoenzymatic synthesis of size-defined polysaccharides by sialyltransferase-catalyzed block transfer of oligosaccharides. Submitted.
H. Book Edited
Chemical Glycobiology ACS book series, Chen, X.; Halcomb, R.; Wang, P. G. Eds. In progress.
I. PATENT APPLICATIONS
1. Wang, P. G.; Chen, X.; Liu, Z. Y.; Zhang, W. Glycoconjugate synthesis using a pathway-engineered organism, UA2002132320.
2. Wang, P. G.; Chen, X. Glycoconjugate and sugar nucleotide synthesis using solid supports, UA2002150968, WO0255723.
3. Chen, X.; Saribas, S.; Barthelson, R.; Mobasseri, A.; Kelly, J.; Otvos-Papp, E.; Pristatsky, P.; Wang, J.; Stevenson, T. T. Manufacture of heparin for anticoagulant using transgenic microorganisms and heparin precursors. PCT Int. Appl. (2004), 118 pp. WO2004017910 A2 20040304.
4. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Glycan remodeling and glycoconjugation of peptides and proteins. PCT Int. Appl. (2003), 900 pp. WO 2003031464 A2 20030417.
5. Defrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Cell-free, in vitro method for regioselective, enzymic glycoPEGylation of peptides. U.S. Pat. Appl. Publ. (2004), 752 pp., Cont.-in-part of Appl. No. PCT/US02/32263. US 2004132640 A1 20040708.
6. Defrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Methods for glycan remodeling and glycoPEGylation of therapeutic proteins. U.S. Pat. Appl. Publ. (2004), 690 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004142856 A1 20040722.
7. Defrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Methods for glycan remodeling and glycoPEGylation of therapeutic proteins. U.S. Pat. Appl. Publ. (2004), 754 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004126838 A1 20040701.
8. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Glycan remodeling and glycoconjugation of b-interferon. U.S. Pat. Appl. Publ. (2004), 761 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004115168 A1 20040617.
9. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Glycan remodeling and glycoconjugation of interferona and use thereof for treatment of hepatitis C. U.S. Pat. Appl. Publ. (2004), 761 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004082026 A1 20040429.
10. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Erythropoietin glycosylation and the modification of protein structure and activity for therapeutic use. PCT Int. Appl. (2004), 1018 pp. WO 2004033651 A2 20040422.
11. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Glycan remodeling and glycoconjugation of granulocyte colony stimulating factor. U.S. Pat. Appl. Publ. (2004), 754 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004077836 A1 20040422.
12. DeFree, S.; Zopf, D.; Bayer, R.; Hakes, D.; Chen, X. Remodeling of protein-linked oligosaccharide moieties and the resulting glycoproteins and glycopeptides. U.S. Pat. Appl. Publ. (2004), 749 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2004063911 A1 20040401.
13. DeFrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Methods for cell-free remodeling and glycoconjugation of glycopeptides, remodeling of a-galactosidase A peptides, and their therapeutic use for Fabry disease. U.S. Pat. Appl. Publ. (2004), 761 pp., Cont.-in-part of Appl. No. PCT/US02/32263. US 20040043446 A1 20040304.
14. De Frees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Cell-free oligosaccharide remodeling and glycoPEGylation methods and the proteins/peptides produced. PCT Int. Appl. (2004), 1024 pp. WO 2004099231 A2 20041118.
15. Defrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Cell-free in vitro glycoconjugation of interleukin 2 as therapeutic agent against cancer and AIDS in mammal and human. U.S. Pat. Appl. Publ. (2005), 750 pp., Cont.-in-part of U.S. Ser. No. 360,779. US 2005031584 A1 20050210.
16. Defrees, S.; Zopf, D.; Bayer, R.; Bowe, C.; Hakes, D.; Chen, X. Cell-free in vitro glycan remodeling and enzymic glycoconjugation of Factor IX for treating hemophilia B. U.S. Pat. Appl. Publ. (2005), 761 pp., Cont.-in-part of U. S. Ser. No. 360,779. US 2005100982 A1 20050512.
J. LICENSED TECHNOLOGY
1. Chen, X.; Wang, P. G.; Zhang, Y. N.; Brew, K. “A highly efficient recombinant a2,3-galactosyltransferase”, April 1998.
1. Oct. 27-30, 2004 Speaker, The 39th ACS Western Regional Meeting, Sacramento, CA.
“Synthesis and functional analysis of sialoglycoconjugates”
2. Jan. 14-15, 2005 Speaker, The 8th Annual San Diego Glycobiology Symposium, San Diego, CA. “Chemo-enzymatic synthesis of sialosides”
3. March 31, 2005 Seminar speaker, University of California-Davis, Radiodiagnosis and Therapy Laboratories, Sacramento, CA. “Glycoengineered antibodies for cancer therapy”
4. May 4, 2005 Seminar Speaker, Department of Biochemistry, Ohio State University, Columbus, OH. “Chemo-enzymatic synthesis of sialosides and sialoglycoconjugates”
5. May 6, 2005 Speaker, Mentoring for the future in academic chemistry – NIH workshop, Greenbelt, MD. “Studies of naturally occurring structurally modified carbohydrates”,
6. June 23, 2005 Poster highlights short talk, Carbohydrate Gordon Research Conference, Tilton, NH, “Combinatorial chemo-enzymatic synthesis of sialosides”
7. July 8, 2005 Speaker, National conference on biotechnology on natural resources and glycotechnology, Jinan, Shandong, China, “Chemo-enzymatic synthesis of sialosides”
8. July 15, 2005 Seminar speaker, Shanghai Institute of Organic Chemistry, Shanghai, China. “Chemo-enzymatic synthesis of sialosides and sialoglycoproteins“
9. August 1, 2005 Speaker, 2005 NSF Organic Chemistry Workshop, Bodega Bay, CA. “Chemo-enzymatic synthesis and application of oligosaccharides and glycoconjugates“,
10. Nov. 16, 2005 Seminar speaker, Recruiting Seminar, California State University at Long Beach “Enzymes in organic synthesis”
11. Jan. 20-21, 2006 Speaker, The 9th Annual San Diego Glycobiology Symposium, San Diego, CA. “Sialyltransferases: structure and function”
12. Feb. 21, 2006 Seminar speaker, Recruiting Seminar, University of the Pacific “Sialyltransferases in the synthesis of carbohydrates”
13. July 7, 2006 Seminar speaker, Department of Chemistry, East China Normal University, Shanghai, China, “One-pot three-enzyme chemoenzymatic synthesis of naturally occurring and unnatural sialosides”
14. July 7-9, 2006 Speaker, The Second Sino-US Chemistry Professor Conference, Shanghai, China, “Chemoenzymatic synthesis and biological studies of sialosides”
15. July 23-28, 2006 Speaker and session chair, XXIIIrd International Carbohydrate Symposium, Whistler, BC Canada, “Structural studies of a multifunctional sialyltransferase from Pasteurella multocida”
16. Aug. 10-13, 2006 Invited poster presenter, Trans-Atlantic Frontiers of Chemistry Symposium, New England Center, Durham, New Hampshire “Combinatorial chemoenzymatic synthesis of carbohydrates”
17. Aug. 25-26, 2006 Invited poster presenter, Beckman Foundation Symposium, the Beckman Center of the National Academies of Sciences and Engineering, Irvine, California, “Chemoenzymatic synthetic strategy for mapping molecular recognition of carbohydrate-binding proteins”
18. Sept. 10-14, 2006 Speaker and Organizer, Chemical Glycobiology Symposium, 232nd National Meeting of the American Chemical Society, San Francisco, CA. “Chemoenzymatic synthesis of sialosides and their derivatives”
19. Oct. 27-28, 2006 Invited poster presenter, UC Davis Research Retreat on “Host Microbe Interaction”, “High-throughput substrate specificity studies of sialidases using chemoenzymatically synthesized sialoside libraries”
20. Dec. 17-21, 2006 Speaker, The 9th International Symposium for Chinese Organic Chemists (ISCOC-9), Singapore. “Enzymes in the organic synthesis of sialosides”
21. Dec. 29, 2006 Seminar speaker at Xiamen University, China, “Chemoenzymatic synthesis and functional studies of carbohydrates“
22. Jan. 19-20, 2007 Speaker, The 10th Annual San Diego Glycobiology Symposium, San Diego, CA. “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”
23. Feb. 9, 2007 Seminar speaker, Department of Chemistry and Biochemistry, UC Santa Barbara, “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”
24. Feb. 14, 2007 Seminar speaker, Department of Chemistry, Wayne State University “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”,
25. Feb. 19, 2007 Seminar speaker, Department of Chemistry, University of Toledo “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”
26. Feb. 21, 2007 Seminar speaker, Department of Chemistry, West Virginia University “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”,
27. Feb. 22, 2007 Seminar speaker, Department of Chemistry, Ohio State University “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”,
28. March 9, 2007 Speaker, Bryan Miller Symposium at UC Davis “Chemoenzymatic synthesis and functional studies of sialic acid-containing carbohydrates”,
29. March 27-29, 2007 Speaker, CERC3-NSF, Modern Carbohydrate and Glycoconjugate Chemistry Workshop, Dublin, Ireland, “Chemoenzymatic synthesis of sialosides”,
30. April 20, 2007 Speaker, NIGMS Sponsored Workshop “Expanding the Chemical Space for Carbohydrates: Synthetic Strategies, High Throughput Technologies & Standards
31. May 4-5, 2007 Keynote Speaker at the Alberta Ingenuity Center for Carbohydrate Science Annual Meeting/Third Carbohydrate Symposium in Lake Louise, Alberta, Canada, “Carbohydrates with post-glycosylational modifications”
32. May 18, 2007 Seminar speaker, Department of Chemistry, UC Riverside “Towards an understanding of carbohydrate post-glycosylational modifications”
33. June 17-22, 2007 Speaker at Carbohydrate Gordon Research Conference, Tilton, NH “Towards an understanding of sialic acid post-glycosylational modifications”
34. Aug. 16, 2007 Seminar speaker, Department of Chemistry and Biochemistry, UC Los Angeles “Chemoenzymatic approaches to studying carbohydrate recognizing proteins”
35. Aug. 19, 2007 Speaker, Young Academic Investigators Symposium, Organic Division, Fall 2007 ACS meeting, Boston. Aug. 19-23, 2007, 234th National ACS meeting, Boston “Chemoenzymatic synthesis of carbohydrates with natural and non-natural modifications”
36. Aug. 24-25, 2007 Invited poster presenter, Beckman Foundation Symposium, the Beckman Center of the National Academies of Sciences and Engineering, Irvine, California “Chemoenzymatic synthetic strategy for mapping molecular recognition of carbohydrate-binding proteins”
37. Aug. 31, 2007 Seminar speaker, Department of Chemistry, Iowa State University “Chemoenzymatic approaches to studying carbohydrate recognizing proteins”
38. Sept. 6, 2007 Seminar speaker, Department of Medicinal Chemistry, College of Pharmacy, University of Texas, Austin
39. Sept. 11, 2007 Seminar speaker, School of Pharmacy, University of North Carolina
40. Sept. 19, 2007 Seminar speaker, Department of Chemistry, Stanford University
41. Sept. 26, 2007 Seminar speaker, Department of Biochemistry, Emory University
42. Sept. 27, 2007 Seminar speaker, Department of Chemistry and the Complex Carbohydrate Research Center, University of Georgia
43. Sept. 28, 2007 Seminar speaker, Department of Chemistry, Georgia State University
44. Sept. or Oct., 2007 Seminar speaker, Department of Chemistry, UC Berkeley
45. Jan. 14, 2008 Seminar speaker, Department of Chemistry and Biochemistry, UC San Diego
46. Feb. 6, 2008 Seminar speaker, Department of Chemistry, Caltech
47. Feb. 8-9, 2008 The 11th Annual San Diego Glycobiology Symposium, San Diego, CA.
48. Feb. 22-24, 2008 Speaker, International Conference on the Interface of Chemistry-Biology in Biomedical Research (ISCBC-2008), Birla Institute of Technology and Science (BITS), India
49. Feb. 27, 2008 Seminar speaker, Department of Chemistry, UC Irvine
L. RESEARCH SUPPORT
Completed Research Support
1. Title: Site-specific glyco-conjugation: a novel strategy for linking biomacromoelcules
Agency: The Regents of the University of California
My role: PI
Dates: 01/01/2004 – 06/30/2005
Co-PI: none
Status: expired
2. Title: Faculty Research Travel Grant
Agency: The Regents of the University of California
My role: PI
Dates: 07/01/04 – 06/30/05
Co-PI: none
Status: expired
3. Title: Pathogenic microbial sialidases and their carbohydrate ligands
Agency: Mizutani Foundation for Glycoscience
My role: PI
Dates: 04/01/2005 – 03/31/2006
Co-PI: none
Status: expired
4. Title: Enhance cancer cell apoptosis by in situ cross-linking of antibodies
Agency: University of California Cancer Research Coordinating Committee
My role: PI
Dates: 07/01/2005 – 06/30/2006
Co-PI: none
Status: expired
5. Title: 2005-2006 NMR Award, Chemo-enzymatic synthesis and characterization of naturally occurring structurally modified sialosides
Agency: University of California, Davis NMR Facility
My role: PI
Dates: 07/01/2005 – 06/30/2006
Co-Investigators: none
Status: expired
6. Title: Studies of naturally occurring structurally modified carbohydrates
Agency: The Regents of the Univ. of California (Grant to promote extra mural funding)
My role: PI
Dates: 07/01/2005 – 09/30/2006
Co-PI: none
Status: expired
Current Ongoing Research Support
1. Title: Studies of naturally occurring structurally modified carbohydrates
Agency: NIH – 1R01GM076360
My role: PI
Dates: 01/01/2006 – 12/31/2010
Co-Investigators: none
Status: active
2. Title: CAREER: Harnessing enzymes in novel synthesis of natural carbohydrates
Agency: NSF CAREER Award – 0548235
My role: PI
Dates: 01/01/2006 – 12/31/2010
Co-PI: none
Status: active
3. Title: Chemoenzymatic synthetic strategy for mapping molecular recognition of carbohydrate-binding proteins
Agency: Beckman Young Investigator Program, Arnold and Mable Beckman Foundation
My role: PI
Dates: 09/01/2006 – 08/31/2009
Co-PI: none
Status: active
4. Title: Chemical Glycobiology Symposium
Agency: NIH – 1R13AI073074
My role: PI
Dates: 09/11/2006 – 8/31/2007
Co-PI: Peng George Wang, Randall Halcomb
Status: active
5. Title: Neu5Gc and anti-Neu5Gc antibodies for detection of cancer and cancer risk.
(PI: Ajit Varki, UCSD)
Agency: NIH – 1 U01 CA128442-01
My role: Subcontractor
Dates: 07/01/2007 – 06/30/2012
Status: active
6. Title: NMR as a tool for kinetic studies of multifunctional enzymes
Agency: University of California, Davis NMR Facility (2007-2008 NMR Award)
My role: PI
Dates: 07/01/2007 – 06/30/2008
Co-PI: none
Status: active
7. Title: Faculty Research Travel Grant
Agency: The Regents of the University of California
My role: PI
Dates: 07/01/07 – 06/30/08
Co-PI: none
Status: active
8. Title: Orbitrap (Thermo Fisher) High Resolution/High Mass Accuracy Mass Spectrometer (PI: Julie A. Leary, UCDavis)
Agency: NIH PAR-06-093 Shared Instrumentation Grant (SIF) Program.
My role: Co-PI
Dates: 07/01/2007 – 06/30/2008
Status: active
9. Title: 800 MHz NMR Spectrometer, shared instrument for Chemistry Department.
(PI: James B. Ames, UCDavis)
Agency: NSF
My role: Participant/User
Dates: 07/01/2007 – 06/30/2008
Status: active
Pending Research Support
1. Title: Glycan arrays for discovery and validation of human anti-Neu5Gc antibody as cancer biomarkers (PI: Nancy Hurtado-Ziola, Gc-Free, Inc.)
Agency: NIH – SBIR
My role: Co-PI
Dates: 10/01/2007 – 03/31/2008
Status: pending
2. Title: Delivery of doxorubicin-coated gold nanoparticles by long-circulating liposomes for novel anti-breast cancer radiotherapy (PI: Ting Guo, UCDavis)
Agency: DOD Idea Award
My role: Co-PI
Dates: 01/01/2008 – 12/31/2010
Status: pending
3. Title: Neu5Gc glycopeptide vaccination for ovarian cancer (PI: Dennis Carson, UCSD Cancer Center; Partnering PI: Cheryl C. Saenz, UCSD Cancer Center)
Agency: DOD Translational Research Partnership Award
My role: Subawardee
Dates: 04/01/2008 – 3/31/2011
Status: pending