W. David Wilson

W. David Wilson W. David Wilson
Chair and Regents' Professor
Graduate Biophysical Advisor
Biophysical Chemistry

B.S. (1966):  University of North Carolina
Ph.D. (1970) :  Purdue University
Postdoctoral Fellowship (1971) :  Purdue University

Dr. W. David Wilson
Department of Chemistry
Georgia State University
P.O. Box 3965
Atlanta, Georgia 30302-3965

Department Office Phone: 404-413-5500

Phone: 404-413-5503
Lab Phone: 404-413-5504
Fax: 404-413-5505
Office: 540 Natural Science Center

Research Interests

A number of collaborative projects are underway and all of them focus on the structure and interaction properties of nucleic acids.  We are investigating and developing a range of compounds that interact with different sequences and structures of DNA.  We are particularly interested in the design drugs that can inhibit specific organisms by forming complexes in the DNA minor groove at unique sequences and structures of DNA or RNA.  A range of solution and molecular modeling experiments are being conducted on minor groove complexes of compounds that interact with DNA oligomer sequences that mimic selected sequences from the organism to be targeted.  These studies lead to a molecular model of the complex as a computer model, and logical variations of the drug structure to enhance the DNA interactions can be proposed and initially tested in the computer.  We are particularly interested in developing new classes of agents that can interact with DNA as cooperative dimers.  Such compounds have the ability to simultaneously recognize both strands of DNA to significantly enhance interaction strength and specificity.  We are establishing rules for the specific interaction of heterocyclic dimers with DNA and we now have new types of compounds that can recognize a number of DNA sequences with high specificity. A model of a diphenylfuran dication that has undergone clinical testing is shown below.  The structure was obtained by X-ray crystallography by Stephen Neidle and coworkers at the University of London as part of a collaboration on the development of compounds to target the DNA minor groove.

 2,5-bis-(4-amidinophenyl)furan bound in the minor groove of DNAWe are also involved in a number of collaborative efforts to identify and characterize compounds that can bind to the DNA quadruplex that forms from the telomere sequence at the ends of human chromosomes or in some oncogene promoter regions.  Compounds that bind to the telomere quadruplex have the ability to inhibit the telomerase enzyme that extends the telomere to maintain the viability of rapidly dividing cells.  Since many human cancers require telomerase activity, inhibition of this enzyme is a very attractive method to selectively attack cancer cells.  A number of very promising compounds have been discovered and are now under study.  At the same time, we are searching for general principles that can be used in the design of quadruplex targeting compounds.

Our laboratory and the Chemistry Department have excellent equipment and facilities for studies of nucleic acids and their complexes.  We have BIAcore biosensor-surface plasmon resonance instruments, spectroscopy equipment, calorimeters that include isothermal titration and differential scanning instruments, and an outstanding array of NMR spectrometers.  We use Atomic Force microscopy and Mass spectroscopy equipment from the Departmental Core facilities.

All of these projects involve essential collaborations with other scientists at Georgia State University, at other institutes and universities, and in industry..

This image shows 2,5-bis-(4-amidinophenyl)furan bound in the minor groove of DNA. The binding site is composed of the sequence 5'AATT3' (the adenines are magenta, the thymines are orange). DNA minor groove binding compounds, such as this one, are prime candidates for new drugs  The crystal structure was obtained in a collaborative project with Professor Stephen Neidle.



Recent Publications from the Wilson Laboratory:


"Molecular Determinants For DNA Minor Groove Recognition: Design Of A Bis-Guanidinium Derivative Of Ethidium That Is Highly Selective For AT-Rich DNA Sequences." Christian Bailly, Reem K. Arafa, Farial A. Tanious, William Laine, Christèle Tardy, Amélie Lansiaux, Pierre Colson, David W. Boykin and W. David Wilson, Biochemistry, 44, 1941 (2005).

"Extending The Language Of DNA Molecular Recognition By Polyamides: Unexpected Influence Of Imidazole And Pyrrole Arrangement On Binding Affinity And Specificity." Karen L. Buchmueller, A. Staples, C. Howard, S. Horick, P. Uthe, N. Le, K. Cox, Binh Nguyen, K. Pacheco, W. David Wilson and Moses Lee, J. Am. Chem. Soc., 127, 742.

"New Triple-Helix DNA Stabilizing Agents." Lucjan Strekowski, M. Hojjat, E. Wolinska, A. N. Parker, E. Paliakov, T. Gorecki, Farial A. Tanious and W. David Wilson, Bioorg. Med. Chem. Lett., 15, 1097 (2005).

"Design And Synthesis Of An Expanded Porphyrin That Has Selectivity For The C-MYC G-Quadruplex Structure." J. Seenisamy, S. Bashyam, Vijay Gokhale, H. Vankayalapati, Cory L. Grand, Daekyu Sun, Adam Siddiqui-Jain, Nicole Streiner, Kazuo Shin-ya, Elizabeth White, W. David Wilson and Laurence H. Hurley, J. Am. Chem. Soc., 127, 2944 (2005).

"Dications That Target The DNA Minor Groove: Compound Design And Preparation, DNA Interactions, Cellular Distribution And Biological Activity." W. David Wilson, Binh Nguyen, Farial A. Tanious, Amanda Mathis, James Edwin Hall, Chad E. Stephens and David W. Boykin, Curr. Med. Chem. Anticancer Agents, 5, 389.

"Molecular Recognition Of DNA Base Pairs By The Formamido/Pyrrole And Formamido/Imidazole Pairings In Stacked Polyamides." Karen L. Buchmueller, Andrew M. Staples, Peter B. Uthe, Cameron M. Howard, Kimberly A. O. Pacheco, Kari K. Cox, James A. Henry, Suzanna L. Bailey, Sarah M. Horick, Binh Nguyen, W. David Wilson and Moses Lee, Nucleic Acids Res., 33, 912 (2005).

"Synthesis, DNA Affinity, And Antiprotozoal Activity Of Fused Ring Dicationic Compounds And Their Prodrugs." Reem K. Arafa, Reto Brun, Tanja Wenzler, Farial A.Tanious, W. David Wilson, Chad E. Stephens and David W. Boykin, J. Med. Chem., 48, 5480 (2005).

"Design Of A Hairpin Polyamide, ZT65B, For Targeting The Inverted CCAAT Box (ICB) Site In The Multidrug Resistant (MDR1) Gene." Karen L. Buchmueller, Z. Taherbhai, Cameron M. Howard, Suzanna L. Bailey, Binh Nguyen, C. O' Hare, D. Hochhauser, John A. Hartley, W. David Wilson and Moses Lee, ChemBioChem, 6, 1 (2005).

"Out-Of-Shape DNA Minor Groove Binders: Induced Fit Interactions Of Heterocyclic Dications With The DNA Minor Groove." Yi Miao, Michael P. H. Lee, Gary N. Parkinson, Adalgisa Batista-Parra, Mohamed A. Ismail, Stephen Neidle, David W. Boykin and W. David Wilson, Biochemistry, 44, 14701 (2005).

"Dicationic Near-Linear Biphenyl Benzimidazole Derivatives As DNA-Targeted Antiprotozoal Agents." Mohamed A. Ismail, Adalgisa Batista-Parra, Yi Miao, W. David Wilson, Tanja Wenzler, Reto Brun and David W. Boykin, Bioorg. Med. Chem., 13, 6718 (2005).

"Triple-Helix DNA Selective 2-(2-Naphthyl)Quinoline Intercalators." L. Strekowski, A. N. Parker, M. Hojjat, M. Say, O. Zegrocka-Stendel, S. E. Patterson, Farial A. Tanious and W. David Wilson, Acta Pol. Pharm., 61, 70 (2005).

"Chiral Discrimination In Binding Of Enantiomers Of 2-(Aminoalkoxy)-Substituted 4-(2-Thienyl)Pyrimidines And 4,6-Bis(2-Thienyl)Pyrimidines With Duplex DNA". L. Strekowski, M. T. Cegla, V. Honkan, H. Buczak, W. R. Winkeljohn, A. L. Baumstark, W. David Wilson, Bioorg. Med. Chem. Lett., 15, 2720 (2005).

"Telomestatin And Diseleno Sapphyrin Bind Selectively To Two Different Forms Of The Human Telomeric G-Quadruplex Structure." E. M. Rezler, J. Seenisamy, S. Bashyam, M. Y. Kim, E. White, W. David Wilson and Laurence H. Hurley, J. Am. Chem. Soc., 127, 9439 (2005).

"Binding-Linked Protonation Of A DNA Minor Groove Agent." Binh Nguyen, J. Stanek and W. David Wilson, Biophys J., 90, 1319 (2006).

"3D QSAR On A Library Of Heterocyclic Diamidine Derivatives With Antiparasitic Activity." P. Athri, T. Wenzler, P. Ruiz, Reto Brun, David W. Boykin, Richard Tidwell and W. David Wilson, Bioorg. Med. Chem., 14, 3144 (2006).

"DNA Binding Affinity Of Bisguanidine And Bis(2-Aminoimidazoline) Derivatives With In Vivo Antitrypanosomal Activity." Christophe Dardonville, Michael P. Barrett, Reto Brun, M. Kaiser, F. Tanious and W. David Wilson, J. Med. Chem., 49, 3748 (2006).

"Trisubstituted Acridines As G-Quadruplex Telomere Targeting Agents. Effects Of Extensions Of The 3,6- And 9-Side Chains On Quadruplex Binding, Telomerase Activity, And Cell Proliferation." M. J. Moore, C. M. Schultes, J. Cuesta, F. Cuenca, M. Gunaratnam, Farial A. Tanious, W. David Wilson and Stephen Neidle, J. Med. Chem., 49, 582 (2006).

"Binding Of Netropsin And 4,6-Diamidino-2-Phenylindole To An A2T2 DNA Hairpin: A Comparison Of Biophysical Techniques." M. W. Freyer. R. Buscaglia, Binh Nguyen, W. David Wilson and Edwin A. Lewis, Anal. Biochem., 355, 259 (2006).

"A High-Throughput, High-Resolution Strategy For The Study Of Site-Selective DNA Binding Agents: Analysis Of A Highly Twisted Benzimidazole-Diamidine." K. D. Goodwin, M. A. Lewis, Farial A. Tanious, Richard R. Tidwell, W. David Wilson, Millie M. Georgiadis and Eric C. Long, J. Am. Chem. Soc., 128, 7846 (2006).

"Synthesis And Evaluation Of An Intercalator-Polymide Hairpin Designed To Target The Inverted CCAAT Box 2 In The Topoisomerase Iialpha Promoter." L. V. Flores, A. M. Staples, H. Mackay, C. M. Howard, P. B. Uthe, J. S. Sexton, K. L. Buchmueller, W. David Wilson, C. O’Hare, J. Kluza, D. Hochhauser, J. A. Hartley and Moses Lee, ChemBioChem, 7, 1722 (2006).

"Dicationic DNA-Targeted Antiprotozoal Agents: Naphthalene Replacement of Benzimidazole." S. Chackal-Catoen, Yi Miao, W. David Wilson, T. Wenzler, Reto Brun and David W. Boykin, Bioorg. Med. Chem., 14, 7434 (2006).

"Synthesis, DNA Affinity, And Antiprotozoal Activity Of Linear Dications: Terphenyl Diamidines And Analogues." Mohamed A. Ismail, Reem K. Arafa, Reto Brun, T. Wenzler, Yi Miao, W. David Wilson, C. Generaux, A. Bridges, J. Ed Hall and David W. Boykin, J. Med. Chem., 49, 5324 (2006).

"Synthesis And Antiparasitic Evaluation Of Bis-2,5-[4-Guanidinophenyl]Thiophenes." J. L. Gonzalez, C. E. Stephens, T. Wenzler, R. Brun, Farial A. Tanious, W. David Wilson, T. Barszcz, K. A. Werbovetz and David W. Boykin, Eur. J. Med. Chem., 42, 552 (2007).

"Binding Of Netropsin To Several DNA Constructs: Evidence For At Least Two Different 1:1 Complex Formed From An –AATT-Containing DNA Construct And A Single Minor Groove Binding Ligand." Matt W. Freyer, R. Buscaglia, D. Cashman, S. Hyslop, W. David Wilson, J. Brad Chaires and Edwin A. Lewis, Biophys. Chem., 126, 186 (2007).

"Diphenyl Furans And Aza Analogs: Effects Of Structural Modification On In Vitro Activity, DNA Binding, And Accumulation And Distribution In Trypanosomes." A. M. Mathis, A. S. Bridges, Mohamed A. Ismail, A. Kumar, I. Francesconi, M. Anbazhagan, Q. Hu, Farial A. Tanious, T. Wenzler, J. Saulter, W. David Wilson, R. Brun, David W. Boykin, Richard R. Tidwell and J. Ed Hall, Antimicrob Agents Chemother., 51, 2801 (2007).

"Biosensor - Surface Plasmon Resonance: Quantitative Analysis of Small Molecule - Nucleic Acid Interactions." Binh Nguyen, Farial A. Tanious and W. David Wilson, Methods, 42, 150 (2007).

"Synthesis And Biophysical Evaluation Of Minor-Groove Binding C-Terminus Modified Pyrrole And Imidazole Triamide Analogs Of Distamycin." T. Brown, Z. Taherbhai, J. Sexton, A. Sutterfield, M. Turlington, L. Stallings, K. Buchmueller, H. Mackay H, C. O'Hare, Binh Nguyen, W. David Wilson, Moses Lee and J. A. Hartley, Bioorg. Med. Chem., 15, 474 (2007).

"Unusually Strong Binding To The DNA Minor Groove By A Highly Twisted Benzimidazole-Diphenylether: Induced Fit And Bound Water." Farial A. Tanious, William Laine, Paul Peixoto, Christian Bailly, Kristie D. Goodwin, Mark A. Lewis, Eric C. Long, Millie M. Georgiadis, Richard R. Tidwell and W. David Wilson, Biochemistry, 46, 6944 (2007).

"Induced Fit Conformational Changes Of A 'Reversed Amidine' Heterocycle: Optimized Interactions In A DNA Minor Groove Complex." Manoj Munde, M. Lee, Stephen Neidle, Reem K. Arafa, David W. Boykin, Yang Liu, Christian Bailly and W. David Wilson, J. Am. Chem. Soc., 129, 5688 (2007).

"Break In The Heat Capacity Change At 303 K For Complex Binding Of Netropsin To AATT Containing Hairpin DNA Constructs." M. W. Freyer, R. Buscaglia, A. Hollingsworth, J. Ramos, M. Blynn, R. Pratt, W. David Wilson and E. A. Lewis, Biophys. J., 92, 2516 (2007).

"Structure-Specific Recognition Of Quadruplex DNA By Organic Cations: Influence Of Shape, Substituents And Charge." Elizabeth W. White, Farial Tanious, Mohamed A. Ismail, Anthony P. Reszka, Stephen Neidle, David W. Boykin and W. David Wilson, Biophysical Chemistry, 126, 140 (2007).

"High Affinity Homologous Peptide Nucleic Acid Probes For Targeting A Quadruplex Forming Sequence From A MYC Promoter Element." Subhadeep Roy, Farial Tanious, W. David Wilson, Danith H. Ly and Bruce A. Armitage, Biochemistry, 46, 10433 (2007).

"Sequence Recognition In The Minor Groove Of DNA By Covalently Linked Formamidoimidazole-Pyrrole-Imaidazole Polyamides: The Effect Of H-Pin Linkage And Linker Length On Selectivity And Affinity." C. C. O'Hare, P. Uthe, Hilary Mackay, Kevin Blackmon, Justin Jones, Toni Brown, Binh Nguyen, W. David Wilson, Moses Lee and John Hartley, Biochemistry, 46, 11661 (2007).

"Sequence And Length Dependent Thermodynamic Differences In Heterocyclic Diamidine Interactions At AT Base Pairs In The DNA Minor Groove." Yang Liu, A. Kumar, David. W. Boykin and W. David Wilson, Biophys. Chem., 131, 1 (2007).

"Design Of DNA Minor Groove Binding Diamidines That Recognize GC Base Pair Sequences: A Dimeric-Hinge Interaction Motif." Manoj Munde, Mohamed A. Ismail, Reem K. Arafa, Paul Peixoto, Catherine J. Collar, Yang Liu, Laixing Hu, M. H. David-Cordonnier, A. Lansiaux, Christian Bailly, David W. Boykin and W. David Wilson. J. Am. Chem. Soc., 129, 13732 (2007).

"DNA Minor Groove Interactions Of Antiparasitic Diamidines: Reevaluation Of The Crescent-Shape Concept In Groove-Binding." Binh Nguyen, David W. Boykin and W. David Wilson in Synthetic and Biophysical Studies of DNA Binding Compounds (Editors: Moses Lee and Lucjan Strekowski) (2007).

"Biosensor-Surface Plasmon Resonance Methods For Quantitative Analysis Of Biomolecular Interactions." Farial A. Tanious, Binh Nguyen and W. David Wilson, Methods Cell Biol., 84, 53 (2008).

"Targeting The Inverted CCAAT Box-2 Of The Topoisomerase Iialpha Gene: DNA Sequence Selective Recognition By A Polyamide-Intercalator As A Staggered Dimer." H. Mackay, T. Brown, J. S. Sexton, M. Kotecha, B. Nguyen, W. D. Wilson, J. Kluza, B. Savic, C. O'Hare, D. Hochhauser, M. Lee and J. A. Hartley, Bioorg. Med. Chem.,16, 2093 (2008).

"Synthesis And Antiprotozoal Activity Of Novel Bis-Benzamidino Imidazo[1,2-A]Pyridines And 5,6,7,8-Tetrahydro-Imidazo[1,2-A]Pyridines." M. A. Ismail, R. K. Arafa, T. Wenzler, R. Brun, F. A. Tanious, W. D. Wilson and D. W. Boykin, Bioorg. Med. Chem., 16, 683 (2008).

"Azaterphenyl Diamidines As Antileishmanial Agents." Laixing Hu, Reem K. Arafa, Mohamed A. Ismail, Tanja Wenzler, Reto Brun, Manoj Munde, W. David Wilson, Sandra Nzimiro, Serene Samyesudhas, Karl A. Werbovetz and David W. Boykin, Bioorg. Med. Chem. Lett., 18, 247 (2008).

"Inhibition Of HMGA2 Binding To DNA By Netropsin: A Biosensor-Surface Plasmon Resonance Assay." Yi Miao, Tengjiao Cui, Fenfei Leng, and W. David Wilson, Anal. Biochem., 374, 7 (2008).

"New Bis(2-Aminoimidazoline) And Bisguanidine DNA Minor Groove Binders With Potent In Vivo Antitrypanosomal And Antiplasmodial Activity." Fernando Rodriguez, Isabel Rozas, Marcel Kaiser, Reto Brun, Binh Nguyen, W. Wilson, Rory Garcia and Christophe Dardonville, J. Med. Chem., 51, 909 (2008).

"Antitumor Polycyclic Acridines. 20. Search For DNA Quadruplex Binding Selectivity In A Series Of 8,13-Dimethylquino[4,3,2-kl]acridinium Salts: Telomere- Targeted Agents." M. K. Cheng, C. Modi, J. C. Cookson, I. Hutchinson, R. A. Heald, A. J. McCarroll, S. Missailidis, F. Tanious, W. D. Wilson, J. L. Mergny, C. A. Laughton and M. F. Stevens, J. Med. Chem., 51, 963 (2008).

"Synthesis Of 4,8-Substitiutednaphtho[2,1-b]Thiophene Threading Intercalators." Sahar Badra, Mohamed M. El-Kerdawy, Farial A.Tanious, W. David Wilson and David W. Boykin, J. Heterocyclic Chem., 45, 123 (2008).

"Tri- And Tetra-Substituted Naphthalene Diimides As Potent G-Quadruplex Ligands." Francisco Cuenca, Olga Greciano, Mekala Gunaratnam, Shozeb Haider, Deeksha Munnur, Rupesh Nanjunda, W. David Wilson and Stephen Neidle, Bioorganic & Medicinal Chemistry Letters, 18, 1668 (2008).

"Antiparasitic Compounds That Target DNA." W. David Wilson, Farial A. Tanious, Amand Mathis, Denise Tevis, James Edwin Hall and David W. Boykin, Biochimie,90, 999 (2008).

"Submicromolar Selective G-Quadruplex Ligands From One Pot: Thermodynamic And Structural Studies Of Human Telomeric DNA Binding By Azacyanines." Özgül Persil Çetinkol, Aaron E. Engelhart, Rupesh K. Nanjunda, W. David Wilson, and Nicholas V. Hud, ChemBioChem, 9, 1889 (2008).

"Direct Inhibition Of The DNA Binding Activity Of POU Transcription Factors Pit-1 And Brn-3 By Selective Binding Of A Phenyl-Furan-Benzimidazole Dication." Paul Peixoto, Yang Liu, Sabine Depauw, M. Hildebrand, David W. Boykin, Christian Bailly, W. David Wilson and Marie-Hélène David-Cordonnier, Nucleic Acids Research, 36, 3341 (2008).

"Novel Linear Triaryl Guanidines, N-Substituted Guanidines and Potential Prodrugs as Antiprotozoal Agents." Reem K Arafa, Mohamed A Ismail, Manoj Munde, W. D Wilson, Tanja Wenzler, Reto Brun and David W. Boykin, Eur. J. Med. Chem.,43, 2901 (2008).

"Modifying The N-Terminus of Polyamides: PyImPyIm Has Improved Sequence Specificity Over f-ImPyIm." Toni Brown, Hilary Mackay, Mark Turlington, Arden Sutterfield, Traci Smith, Alan Sielaff, Laura Westrate, Chrystal Bruce, Jerome Kluza, Caroline O'Hare, Binh Nguyen, W. David Wilson, John A. Hartley and Moses Lee. Bioorg. Med. Chem., 16, 5266 (2008).

"Influence Of DNA Structure On Adjacent Site Cooperative Binding." M. Rahimian, Y. Miao and W. D. Wilson, J. Phys. Chem. B., 112, 8770 (2008).

“Rational Design Of Substituted Diarylureas: A Scaffold For Binding To G-Quadrupled Motifs” W. C. Drewe, R. Nanjunda, M. Gunaratnam, M. Beltran, G. N. Parkinson, A. P. Reszka, W. D. Wilson, S. Neidle, J. Med. Chem., 51, 7751 (2008).

"Heterocyclic Diamidine Interactions At AT Base Pairs In The DNA Minor Groove: Effects Of Heterocycle Differences, DNA AT Sequence And Length." Y. Liu, C. J. Collar, A. Kumar, C. E. Stephens, D. W. Boykin and W. D. Wilson, J. Phys. Chem. B., 112, 11809 (2008).

"Sequence Specific And High Affinity Recognition Of 50-ACGCGT-30 By Rationally Designed Pyrrole-Imidazole H-Pin Polyamides:Thermodynamic And Structural Studies." H. Mackay, T. Brown, P. B. Uthe, L. Westrate, A. Sielaff, J. Jones, J. P. Lajiness, J. Kluza, C. O’Hare, B. Nguyen, Z. Davis, C. Bruce, W. D. Wilson, J. A. Hartley and M. Lee, Bioorganic Med. Chem., 16, 9145 (2008).

"Synthesis Of Acridine Based Threading Intercalators." Sahar Badra, Mohamed M. El-Kerdawy, Farial A.Tanious, W. David Wilson and David W. Boykin, J. Heterocyclic Chem, 14, 15, (2008).

"A Role For Water Molecules In DNA-Ligand Minor Groove Recognition." B. Nguyen, S. Neidle and W. D. Wilson, Acc. Chem. Res., 42, 11, (2009).

“Minor Groove Binding Compounds That Jump A GC Base Pair And Bind To Adjacent AT Base Pair Sites.” M. Rahimian, A. Kumar, M. Say, S. A. Bakunov, D. W. Boykin, R. R. Tidwell, W. D. Wilson, Biochemistry, 48, 1573, (2009).

“Large, Sequence-Dependent Effects On DNA Conformation By Minor Groove Binding Compounds.” D. S. Tevis, A. Kumar, C. E. Stephens, D. W. Boykin, W. D. Wilson, Nucleic Acids Res., 37, 5550, (2009).

“Molecular Dynamics Of Water-Mediated Interactions Of A Linear Benzimidazole-Biphenyl Diamidine With The DNA Minor Groove.” P. Athri, W. D. Wilson, J. Am. Chem. Soc. 131, 7618, (2009) .

“Polyamide Curvature And DNA Sequence Selective Recognition: Use Of 4-Aminobenzamide To Adjust Curvature.” J. Lajiness, A. Sielaff, H. Mackay, T. Brown, J. Kluza, B. Nguyen, W. D. Wilson, M. Lee, J. A. Hartley, Med. Chem. 5, 216, (2009).

“Effects Of The N-Terminal Acylamido Group Of Imidazole- And Pyrrole-Containing Polyamides On DNA Sequence Specificity And Binding Affinity.” L. Westrate, H. Mackay, T. Brown, B. Nguyen, J. Kluza, W. D. Wilson, M. Lee, J. A. Hartley, Biochemistry 48, 5679, (2009).

“Crystal Structure Of A Trypanocidal 4,4'-Bis(Imidazolinylamino)Diphenylamine Bound To DNA.” L. S. Glass, B. Nguyen, K. D. Goodwin, C. Dardonville, W. D. Wilson, E. C. Long, M. M. Georgiadis, Biochemistry 48,5943, (2009).

“Synthesis And Activity Of Azaterphenyl Diamidines Against Trypanosoma Brucei Rhodesience And Plasmodium Falciparum.” L. Hu, R. K. Arafa, M. A. Ismail, A. Patel, M. Munde, W. D. Wilson, T. Wenzler, R. Brun, D. W. Boykin, Bioorganic and Medicinal Chemistry 17, 6651, (2009).

“Predictive Computational Models Of Substrate Binding By A Nucleoside Transporter.” Catharine J. Collar, Mohammed Al-Salabi, Mhairi L. Stewart, Michael P. Barrett, W. David Wilson, Harry P. de Koning, J. Biol. Chem. 284, 34028, (2009).

“The Effects of Hairpin loops on Ligand-DNA Interactions.” Binh Nguyen, W. David Wilson, The Journal of Physical Chemistry B, 113, 14329, (2009).

"Loop and Backbone Modifications of PNA Improve G-Quadruplex Binding Selectivity." Sabrina Lusvarghi; Connor Murphy, Subhadeep Roy, Farial Tanious, Iulia Sacui, W. Wilson, Danith Ly, Bruce Armitage, J. Am. Chem. Soc., 131, 18415, (2009).

"Synthesis, DNA Binding, Fluorescence Measurements And Antiparasitic Activity Of DAPI Related Diamidines." A. A. Farahat, A. Kumar, M. Say, A. E. Barghash, F. E. Goda, H. M. Eisa, T. Wenzler, R. Brun, Y. Liu, L. Mickelson, W. D. Wilson, D. W. Boykin, Bioorganic Med. Chem., 18, 557, (2010).

“Quantitative Analysis Of Small Molecule-nucleic Acid Interactions With A Biosensor Surface And Surface Plasmon Resonance Detection.” Y. Liu, W. D. Wilson, Methods Mol. Biol., 613, 1, (2010).

“A Selective TrkB Agonist With Potent Neurotrophic Activities By 7,8-Dihydroxyflavone.” S. W. Jang, X. Liu, M. Yepes, K. R. Shepherd, G. W. Miller, Y. Liu, W. D. Wilson, G. Xiao, B. Blanchi, Y. E. Sun, K. Ye, Proc. Natl. Acad. Sci. U S A., 107, 2687, (2010).

“Synthesis And Antiprotozoal Activity Of 2,5-bis[amidinoaryl]thiazoles.” D. Branowska, A. A. Farahat, A. Kumar, T. Wenzler, R. Brun, Y. Liu, W. D. Wilson, D. W. Boykin, Bioorg. Med. Chem. 18, 3551, (2010).

“Novel Arylimidamides For The Treatment Of Visceral Leishmaniasis.” M. Z. Wang, X. Zhu, A. Srivastava, Q. Liu, J. M. Sweat, T. Pandharkar, C. E. Stephens, E. Riccio, T. Parman, M. Munde, S. Mandal, R. Madhubala, R. R. Tidwell, W. D. Wilson, D. W. Boykin, J.E. Hall, D. E. Kyle, K. A. Werbovetz, Antimicrob. Agents Chemother 54, 2507, (2010).

“DNA Minor Groove Induced Dimerization Of Heterocyclic Cations: Compound Structure, Binding Affinity, And Specificity For A TTAA Site.” M. Munde, A. Kumar, R. Nhili, S. Depauw, M. H. David-Cordonnier, M. A. Ismail, C. E. Stephens, A. A. Farahat, A. Batista-Parra, D. W. Boykin, W. D. Wilson, J. Mol. Biol. 402, 847, (2010).

“Setting Anchor In The Minor Groove: In Silico Investigation Into Formamido N-Methylpyrrole And N-Methylimidazole Polyamides Bound By Cognate DNA Sequences.” C. J. Collar, M. Lee, W. D. Wilson, J. Chem. Inf. Model 50, 1611, (2010).

“Diamidines For Human African Trypanosomiasis.” M. F. Paine, M. Z. Wang, C. N. Generaux, D. W. Boykin, W. D. Wilson, H. P. De Koning, C. A. Olson, G. Pohlig, C. Burri, R. Brun, G. A. Murilla, J. K. Thuita, M. P. Barrett, R. R. Tidwell, Curr. Opin. Investig. Drugs 8, 876, (2010).

“DNA Recognition: Design, Synthesis And Biophysical Characteristics Of Pyrrole(H) Based Polyamides.” S. Chavda, K. Mulder, T. Brown, H. Mackay, B. Babu, L. Westrate, A. Ferguson, S. Lin, K. Kiakos, J. P. Ramos, M. Munde, W. D. Wilson, J. A. Hartley, M. Lee, Med. Chem. 6, 150, (2010).

“Targeting The Icb2 Site Of The Topoisomerase Iialpha Promoter With A Formamido-Pyrrole-Imidazole-Pyrrole H-Pin Polyamide.” A. Franks, C. Tronrud, K. Kiakos, J. Kluza, M. Munde, T. Brown, H. Mackay, W. D. Wilson, D. Hochhauser, J. A. Hartley, M. Lee, Bioorg. Med. Chem. 18, 5553, (2010).

“Understanding The DNA Binding Of Novel Non-Symmetrical Guanidinium/2 Aminoimidazolinium Derivatives.” P. S. Nagle, S. J. Quinn, J. M. Kelly, D. H. O'Donovan, A. R. Khan, F. Rodriguez, B. Nguyen, W. D. Wilson, I. Rozas, Org. Biomol. Chem. 8, 5558, (2010).

“Pharmacophore Model For Pentamidine Analogs Active Against Plasmodium Falciparum.” P. Athri, T. Wenzler, R. Tidwell, S. M. Bakunova, W. D. Wilson, Eur. J. Med. Chem. 45, 6147, (2010).

“Induced Topological Changes In DNA Complexes: Influence Of DNA Sequences And Small Molecule Structures.” Rebecca A. Hunt, Manoj Munde, Arvind Kumar, Mohamed A. Ismail, Abdelbasset A. Farahat, Reem K. Arafa, Martial Say, Adalgisa Batista-Parra, Denise Tevis, David W. Boykin, W. David Wilson, Nucleic Acids Research In Press 2011.

“Dicationic Phenyl-2,20-bichalcophenes And Analogues As Antiprotozoal Agents.” Mohamed A. Ismail, Serry A. El Bialy, Reto Brun, Tanja Wenzler, Rupesh Nanjunda, W. David Wilson, David W. Boykin, Bioorganic & Medicinal Chemistry 19, 978, (2011).

“Surface Plasmon Resonance And Quartz Crystal Microbalance Methods For Detection Of Introduction Molecular Interactions.” Yang Liu, Archana Jaiswal, Mark A. Poggi, W. David Wilson, Eds. Binghe Wang and Eric V. Anslyn, John Wiley and Sons (2011).

“Hx, A Novel Fluorescent, Minor Groove And Sequence Specific Recognition Element: Design, Synthesis, And DNA Binding Properties Of p-Anisylbenzimidazole-imidazole/pyrrole-Containing Polyamides.” S. Chavda, Y. Liu, B. Babu, R. Davis, A. Sielaff, J. Ruprich, L. Westrate, C. Tronrud, A. Ferguson, A. Franks, S. Tzou, C. Adkins, T. Rice, H. Mackay, J. Kluza, S. A. Tahir, S. Lin, K. Kiakos, C. D. Bruce, W. D. Wilson, J. A. Hartley, M. Lee, Biochemistry 50, 3127, (2011).   

Current Research Grant Support:


National Institutes of Health:  GM-61587;

"Sequence-Specific Recognition of DNA by a Dimer Motif."


National Institutes of Health:  AI-064200;

"Heterocycle Binding and Biology in the DNA Minor Groove."


Bill and Melinda Gates Foundation

“Development of New Drug Candidates for Treatment of Human African Trypanosomiasis and Leishmaniasis.”


National Science Foundation:  CHE-0809162

"Self-assembly and Programmable Nuclear Permeable Polyamides That Target Specific DNA Sequences with Slow Off-rates and High Affinity: Development of a Molecular Toolkit”



Greater than 20 US Patents concerning minor groove binding heterocyclic cations.