Markus W. Germann

Markus W. Germann Markus W. Germann
Safety Director, Professor
GCC Distinguished Scholar
Biophysical Chemistry

M.S. (1983) :  Technikum Winterthur Ingenieurschule
Ph.D. (1989) :  University of Calgary
Postdoctoral Scientist (1989-1990) :  University of Calgary

Dr. Markus W. Germann
Department of Chemistry
Georgia State University
P.O. Box 3965
Atlanta, Georgia 30302-3965

Department Office Phone: 404-413-5500

Phone: 404-413-5561
Lab Phone: 404-413-5523
Fax: 404-413-5505
Office: 413 Natural Science Center


Featured Scholar article from the Office of Vice President for Research.

The major focus of our research is the determination of structure, dynamics and function of biological macromolecules by high resolution NMR spectroscopy and molecular dynamics simulations.

Research Interests 
DNA damage: Effect on DNA structure and repair/recognition by repair enzymes. 
Unusual DNA constituents: To study and control DNA stability, recognition and dynamics. 
HIV- RNA binding Zinc fingers: Design, structure and combinatorial methods to generate small RREIIB RNA binding zinc fingers that inhibit rev binding and virus production. 
NMR imaging and Metabonomics.

Methods and Techniques in use 
DNA/RNA synthesis/purification and characterization 
NMR spectroscopy for characterization and 3D-structure determination 
Cloning to generate new mutants and protein expression systems, Phage Display 
Protein production (in vivo and in vitro), purification 
Div. Enzymatic assays, PAGE 
Circular Dichroism, Fluorescence, UV spectroscopy, UV melting studies Surface plasmon resonance 
Ab initio and molecular dynamics calculations

1982    M.S.    Chemical Engineering Technikum Winterthur, Switzerland
1989    Ph.D.    Nucleic Acids Biochemistry University of Calgary, Canada
1990    Post Doc.    Physical Biochemistry University of Calgary, Canada


Professional Experience
1975-1978    Apprenticeship, Laborant B, Ciba-Geigy AG, Basel, Switzerland
1978-1979    Research Technician, Ciba-Geigy AG, Basel, Switzerland
1979-1983    Chemiker HTL, Ciba-Geigy AG, Basel, Switzerland (B.S. Chemical Engineering)
1983-1989    Graduate Student, The University of Calgary, Alberta, Canada
1989-1990    Postdoctoral Fellow, laboratory of Dr. H. J. Vogel, The University of Calgary, Canada
1991-1993    Application Specialist, Bruker-Spectrospin AG, Switzerland
1994-2001    Assistant Professor, Thomas Jefferson University, Kimmel Cancer Institute, Departments of Microbiology and Immunology, Philadelphia, PA
2001 Associate Professor, (Kimmel Cancer Institute and Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia USA
2001- Associate Professor, Department of Chemistry, Georgia State University, Atlanta, USA


Representative Publications

Hamilton, C. J & Germann, M. W.: “Folding and Base Pairing of a Fibrinogen Specific DNA Aptamer. Colonial Academic Alliance Undergraduate Research Journal, (2011), Vol. 2, Art 11.

Germann, M. W. Subrata, M. H., Santoso, Y. & Spring, A. M.: “Designing HIV-RREIIB RNA Binding Zinc Fingers” In Zinc Fingers: Structure, Properties and Applications, (2011) Nova Science Publishers, Inc. ISBN 978-1-62100-230-7.

Kicklighter, C. E., Kamio, M., Nguyen, L., Germann, M. W. & Derby, C. E.: An economy of chemicals: sea hare use sun-screening mycosporine-like amino acids as intraspecific alarm cues. PNAS (2011), 108, 11494-11499.

Zhang, J. & Germann, M. W.: Characterization of Secondary Amide Peptide Bonds Isomerization: Thermodynamics and Kinetics from 2D NMR Spectroscopy. Biopolymers, (2011), 755-762.

Johnson, C. N., Spring, A. M., Shaw, B. R & Germann, M. W.: Structural Basis of the RNase H1 Activity on Stereo Regular Borano Phosphonate DNA / RNA Hybrids. Biochemistry, (2011), 50, 3903-3912.

Kamio, M., Kicklighter, C. E., Nguyen, L., Germann, M. W. & Derby, C. E.: Isolation and Structural Elucidation of Novel Mycosporine-like Amino Acids as Alarm Cues in the Defensive Ink Secretion the Sea Hare Aplysia californica, Helvetica Chimica Acta, (2011), 94, 1012-1018.

Herberholz, J., Mishra, S., Germann, M. W., Edwards, D. H. & Potter, K.: Non-invasive imaging of neuroanatomical structures and neural activity at high spatial resolution. Frontiers in Behavioral Neuroscience, (2011), 5, 16, 1-9.

Zhang, X., Dou, J. & Germann, M. W.: Characterization of the cellular immune response in hepatitis C virus infection. Methods and Technologies. Mini-Reviews in Medicinal Chemistry (2011), 11, 106-113.

Germann, M.W., Johnson, C. N. & Spring, A.M.: Structure and Dynamic Markers in DNA Damage Recognition. 2010, Medicinal Research Reviews (2010)

Tang, Z., Xu, B., Wu, B., Germann M.W. & Wang, G.: Synthesis and Structural Determination of Multidentate 2,3-Dithiol-Stabilized Au Clusters. J. Am. Chem. Soc. (2010), 132, 3367–3374.

Khan, A.M., Subrata, M. & Germann, M. W.: Cyclic enzymatic solid phase synthesis of DNA oligonucleotides on an epoxide-activated resin.  Nucleosides, Nucleotides and Nucleic Acids (2009), 28, 1030-1041

Germann, M. W., Johnson, N. J. & Spring, A.M.:  Unusual DNA Structure and DNA Damage Recognition: Structure and Dynamic Markers.  Chimia (2009), 63, 731-736

Mishra, S. M., Spring, A.M. & Germann, M.W.: Thermodynamic profiling of HIV RREIIB RNA - zinc finger interactions.  J. Mol. Biol. (2009), 393, 369-382.

Pei, H., Germann, M.W.  & Allison, S.A.: Translational Diffusion Constants of Short Peptides: Measurement by NMR and their use in structure study of peptides.  Journal of Physical Chemistry B, (2009), 113, 9326-9329.

Zhang, J., Spring, A. & Germann, M.W.:  Facilitated Assignment of Adenine H2 Resonances in Oligonucleotides using Homonuclear Long-range Couplings.  Journal of the American Chemical Society, (2009), 131, 5380–538.

Zhang, X., Dou, J. & Germann, M.W.:  Characterization of the cellular immune response in hepatitis C virus infection.  Medicinal Research Reviews (2009), 29, 843-866.

Horowitz, E. Lilavivat, S. Holladay, B., Germann, M.W. & Hud, N.V.: Solution Structure and Thermodynamics of 2',5' RNA Intercalation. Journal of the American Chemical Society (2009) 131, 5831–5838.

Mazurek, A., Johnson C.N., Germann, M.W. & Fishel, R.: The Role of Nearest Neighbor Sequence Context in Mismatch Recognition by hMSH2-hMSH6. PNAS (2009) 106, 4177-4182.

Di Lullo, G., Reigle, K.L., Turner, K.R. Last, J.A., Birk, D. E., Funderburgh, J.L., Elrod, E., Germann, M.W., Surber, C., Sanderson, R.D. and San Antonio, J.D.: Non-Enzymatic Glycation of Type I Collagen Dimininishes Collagen-Proteoglycan Binding and Weakens Cell Adhesion. Journal of Cellular Biochemistry (2008), 104, 1684-1689.

Kicklighter, C.E., Germann, M.W., Kamio, M. & Derby, C.D.: Molecular Identification of Alarm Cues in the Defensive Secretions if the Sea Hare Aplysis california”.  Animal Behavior (2007), 74, 1481-1492.

Moskau, D., Kovacs, H., Kümmerle, R., Colson, K., Collier, G. & Germann, M.W.: New CryoProbe Models for Diverse Analytical Challenges. SpinReport, (2007), 157, 35-43.

Germann, M.W. Turner, T. & Allison, S.: Translational Diffusion Constants of the Amino Acids:  Measurement by NMR and Their Use in Modeling the Transport of Peptides. Journal of Physical Chemistry A, (2007), 111, 8, 1452-1455.

Voehler, M. Collier, G., Young, J.K., Stone, M.P. & Germann, M.W.:  Performance Of Cryogenic Probes As A Function Of Ionic Strength And Sample Tube Geometry. Journal of Magnetic Resonance (2006), 183, 102-109.

Covarrubias, M., Bhattacharji, A., Harris, T., Kaplan, B. and Germann, M.W.: The structural determinants of alcohol-anesthetic action in the activation gate of a voltage-gated K+ channel. Molecular Pharmacology, (2006), 70, 1542-1554.

Mishra, S.M., Doyle, B., Shelley, C.M., Darby, M. K. and Germann, M.W.: Solution structures and binding of HIV RRE IIB RNA targeting zinc finger proteins. Biopolymers (2006), 83, 352-364.

Fan, F., Germann, M.W. and Gadda, G.: Probing the Chemical Mechanism for Aldehyde Oxidation by Choline Oxidase with Substrate Analogs. Biochemistry (2006), 45, 1979-1986.

Salon, J., Chen, G., Portilla, Y., Germann, M.W. & Huang, Z.:  Synthesis of a 2’-Se-uridine Phosphoramidite and its Incorporation into Oligonucleotides for Structural Study.  Organic Letters (2005), 7, 5645-5648.

Yang, H., Johnson, P. M., Ko, K-C., Kamio, M., Germann, M. W., Derby, C. D., & Tai, P. C. "Escapin, a stable and broadly antimicrobial FAD-containing L-amino acid oxidase from ink of the sea hare Aplysia californica, can be functionally expressed in bacteria", (2005) The Journal of Experimental Biology, 208, 3609-3622.

Vlassiouk, I., Krasnoslobodtsev, A, Smirnov, S. & Germann, M.W. "Direct" Detection and Separation of DNA Using Nanoporous Alumina Filters. (2004) Langmuir, 20 (23), 9913-9915.

Aramini, J.M., Cleaver, S.H., Pon, R.T., Cunningham, R.P. & Germann: Solution Structure of a DNA Duplex Containing an α-Anomeric Adenosine: Insights into Substrate Recognition by Endonuclease IV. (2004), J. Mol. Biol. (2004), 338, 77-91.

Shahidullah, M., Harris, T., Germann, M.W. & Covarubias, M.:  Molecular Features of an Alcohol Binding Site in a Neuronal Potassium Channel (2003), 42, 11243-11252.

Germann, M.W., Aramini, J.M., Kalisch, B.W. & van de Sande, J.H.: Structure, dynamics and enzymatic properties of mixed α,β-oligonucleotides containing polarity reversals. Nucleosides and Nucleotides (2001), 20, 4-7.

Aramini, J.M., Mujeeb, A., Ulyanov, N. B. & Germann, M.W.: Conformational Dynamics in Mixed α,β- Oligonucleotides Containing Polarity Reversals: A Molecular Dynamics Study using Time-averaged Restraints. J. Biomol. NMR, (2000), 18, 287-303.

Verrecchio, A., Germann, M.W. Schick, B. P., Kung, B., Twardowski, T. and San Antonio, J.D.: Design of Peptides with High Affinities for Heparin and Endothelial Cell Proteoglycans, Journal of Biological Chemistry, (2000), 275, 7701-7707.

Aramini, J.M. & Germann, M.W. NMR solution structure of a DNA/RNA hybrid containing an alpha anomeric thymidine and polarity reversals. Biochemistry, (1999), 38, 15448-15458.

Germann, M.W., Kalisch, B.W. & van de Sande, J.H.: Structure of d(GT)nd(GA)nSequences: Formation of Parallel Stranded Duplex DNA. Biochemistry, (1998), 37, 12962-12970.

Aramini, J.M., Germann, M.W. & Vogel, H.J. Soft-Pulsed Aluminum-27 QCT NMR Studies of Ovotransferrin. J. Mag. Res. (1997), 209, 111-114.

Satoh, T., Aramini, J.M., Li, S., Friedman, T., Germann, M.W., Korngold, R. & Huang, Z.: Biologically Active Cyclic Heptapeptide derived from the CC' Loop of human CD4 Domain 1. Journal of Biological Chemistry (1997), 272, 12175-12180.

Aramini, J.M., Kalisch, B. W., Pon, R.T., van de Sande, J.H. & Germann, M.W.: Structure of a DNA duplex that contains alpha anomeric nucleotides and 3'3' and 5'5' phosphodiester linkages:  Coexistence of parallel and antiparallel DNA. Biochemistry (1996), 35, 9355-9365.

Shachar-Hill, Y., Pfeffer, P.E. & Germann, M.W.: In vivo study of plant metabolism using 13C and 15N labeled glucose and ammonia with heteronuclear two dimensional pulsed field gradient spectroscopy. Analytical Biochemistry (1996), 243, 110-118.

Germann, M.W., Zhou, N., van de Sande, J.H. & Vogel, H.J.: Parallel-stranded duplex DNA:  a NMR perspective. Methods in Enzymology (1995), 261, 207-225.

Germann, M.W., Aramini, J.M. & Vogel, H.J.: A Quadrupolar Metal Ion NMR Study of Ovotransferrin at 17.6 Tesla. Journal of the American Chemical Society (1994), 116, 6971-6972.

Aramini, J.M., Germann, M.W. Vogel, & H.J.: Field Dependent Aluminium-27 NMR Studies of the Transferrins: An Approach to the Study of Metal Ion Binding Sites in larger Proteins. Journal of the American Chemical Society (1993), 115, 9750-9753.

Germann, M.W., Kalisch, B.W., Lundberg, P., Vogel, H.J. & van de Sande, J.H.: Perturbation of DNA hairpins containing the EcoRI recognition site by hairpin loops of varying sizes and composition: Physical (NMR & UV) and enzymatic (EcoRI) studies. Nucleic Acids Research (1990), 18, 1489-1498.

Germann, M.W., Vogel, H.J., Pon, R.T. & van de Sande, J.H.: Characterization of a Parallel Stranded DNA Hairpin. Biochemistry (1989), 28, 6220-6228.

van de Sande, J.H., Ramsing, N., Germann, M.W., Elhorst, W., Kalisch, B.W., Pon, R.T. & Jovin, T.M.: Parallel-Stranded Duplex DNA. Science (1988), 241, 551-557.

Spring, A. M. & Germann. M. W.: “Supercooled Aqueous NMR Using Agarose Gels” Analytical Biochemistry. (2012). In press.

Rettig, M., Germann, M.W., Ismail, M. A. Batista-Parra, A., Munde, M., Boykin, D. W. & Wilson, W. D.: “Microscopic Rearrangement of Bound Minor Groove Binders Detected by NMR” Journal of Physical Chemistry B. (2012). In press.

Johnson, C. N., Spring, A. M., Desai, S., Cunningham, R. P. & Germann, M. W.:“DNA sequence context conceals α anomeric lesions.” J. Mol. Biol. (2012) 416, 425-437.