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Stuart A. Allison

Stuart Anthony Allison Stuart Anthony Allison
Professor
Biophysical Chemistry

B.A. (1973):  University of Montana
M.S. (1976) :  University of California at Berkeley
Ph.D. (1980) :  University of Washington
NIH Fellowship (1981-1982) :  University of Oregon

Dr. Stuart Anthony Allison
Department of Chemistry
Georgia State University
P.O. Box 4098
Atlanta, Georgia 30302-4098

Phone: 404-413-5519
Fax: 404-413-5505
Office: 520-A Science Annex
Email:  sallison@gsu.edu

The S. Allison laboratory is primarily interested in biomolecular transport with particular emphasis in the theory of diffusion, viscosity, and electrophoresis. We have focused on detailed modeling of transport with the objective of learning what electrophoresis, diffusion, and viscosity can tell us about biomolecular charge, conformation, and structure. Over the last few years, we have shown that detailed modeling can accurately predict the electrophoretic mobilities of large classes of peptides and, in specific cases, can be used to address conformational questions as well. Modeling of electrophoresis has been carried out both in free solution and in a gel. Modeling in a gel presents particular challenges and it is in that area where current research is concentrated.

RECENT PUBLICATIONS (since 2000)

  1. S.A. Allison and D. Stigter, “A Commentary on the Screened-Oseen, Counterion-Condensation Formalism of Polyion Electrophoresis,” Biophysical Journal, 78, 121-124 (2000).

  2. S. Mazur, C. Chen, and S.A. Allison, “Modeling the Electrophoresis of Short Duplex DNA: Counterions K+ and Tris+,” J. Phys. Chem. B.,105, 1100-1108 (2001) .

  3. S.A. Allison, C. Chen, and D. Stigter, “The Length Dependence of Translational Diffusion, Free Solution Electrophoretic Mobility, and Electrophoretic Tether Force of Rigid Rodlike Model Duplex DNA,” Biophys. J., 81, 2558-2568 (2001).

  4. C. Chen, and S.A. Allison, “Boundary Element Modeling of the Primary Electroviscous Effect of Dilute Sodium Poly(styrenesulfonate) in Monovalent Salt Solutions,” Macromolecules, 34, 8397-8398 (2001).

  5. S. Allison, “Boundary Element Modeling of Biomolecular Transport,”  Biophys. Chem., 93, 197-213 (2001).

  6. S.A. Allison, Z. Li, D. Reed, and N.C. Stellwagen, “Modeling the Gel Electrophoresis of Short Duplex DNA by Brownian Dynamics: Cubic Gel Lattice with Direct Interaction,” Electrophoresis, 23, 2678-2689 (2002).

  7. J.A. Durant, C. Chen, T.M. Laue, T.P. Moody, and S.A. Allison, “Use of T4 Lysozyme Charge Mutants to Examine Electrophoretic Models,”  Biophysical Chemistry, 101-102, 593-609 (2002).

  8. S. Allison, M. Rasmusson, and S. Wall, “The Primary Electroviscous Effect, Free Solution Electrophoretic Mobility, and Diffusion of Dilute Prolate Ellipsoid Particles (Minor Axis = 3 nm) in Monovalent Salt Solution,” J. Colloid Interface Sci. 258, 289-297 (2003). 

  9. M. Rasmusson, S.A. Allison, and S. Wall, “The Primary Electroviscous Effect of Prolate Silica Sols,” J. Colloid Interface Sci. 260, 423-430 (2003).

  10. S. Allison, S. Wall, and M. Rasmusson, “A General Gel Layer Model for the Transport of Colloids and Macroions in Dilute Solution,” J. Colloid Interface Sci. 263, 84-98 (2003).

  11. E.D. Breyer, S. Howard, N. Raje, S. Allison, R. Apkarian, W.V. Brown, and J.K. Strasters, “A New Approach in the Study of Lipid and Apolipoprotein Binding Interactions Using Vesicle Affinity Capillary Electrophoresis, Analytical Chemistry, 75, 5160-5169 (2003).

  12. S.A. Allison, J.D. Carbeck, C. Chen, and F. Burkes, “Electrophoresis of Protein Charge Ladders: A Comparison of Experiment with Various Continuum Primitive Models,” J. Phys. Chem. B., 108, 4516-4524 (2004).

  13. S.A. Allison, “Electrokinetic Phenomena. Principles and Applications in Analytical Chemistry and Microchip Technology.”  (Book Review), J. Amer. Chem. Soc., 126, 6835-6836 (2004).

  14. S.A. Allison, “Analysis of the Electrophoretic Mobility and Viscosity of Dilute Ludox Solutions in Terms of a Spherical Gel Layer Model,” J. Colloid Interface Sci. 277, 248-254 (2004).

  15. S.A. Allison, “Electrophoretic Mobility and Primary Electroviscous Effect of Dilute “Hard” Prolate Ellipsoids,” J. Colloid Interface Sci., 282, 231-237 (2005).

  16. S.A. Allison, Y. Xin, “Electrokinetic Transport of Rigid Macroions in the Thin Double Layer Limit.  A Boundary Element Approach,” J. Colloid Interface Sci., 288, 616-628 (2005).

  17. S.A. Allison, Y. Xin, H. Mitchell, “The Electrophoresis of Weakly Charged Macroions Modeled as Bead Arrays,” in “Recent Research Developments in Macromolecules,” 8 Research Signpost Publishers, (2005) pp. 25-46.

  18. Y. Xin, H. Mitchell, H. Cameron, S.A. Allison, “Modeling the Electrophoretic Mobility and Diffusion of Weakly Charged Peptides,” J. Phys. Chem. B, 110, 1038-1045 (2006).

  19. S.A. Allison, Y. Xin, “Electrokinetic Transport of a Spherical Gel-Layer Model Particle:  Inclusion of Charge Regulation and Application to Polystyrene Sulfonate,”  J. Colloid Interface Sci., 299, 977-988 (2006).

  20. Y. Xin, R. Hess, N. Ho, and S.A. Allison, “Modeling the Electrophoresis of Peptides and Proteins:  Improvements in the “Bead Method” to Include Ion Relaxation and Finite Size Effects,”  J. Phys. Chem. B, 110, 25033-25044 (2006).

  21. S.A. Allison, “Diffusion Controlled Reactions:  Hydrodynamic Interaction Between Charged, Uniformly Reactive Spherical  Reactants,”  J. Phys. Chem. A, 110, 13864-13867 (2006).

  22. M.W. Germann, T. Turner, S.A. Allison, “Translational Diffusion Constants of the Amino Acids:  Measurement by NMR and Their Use in Modeling the Transport of Peptides,” J. Phys. Chem. A, 111, 1452-1457 (2007).

  23. S.A. Allison, Y. Xin, H. Pei, “The Electrophoresis of Spheres with Uniform Zeta Potential in a Gel Modeled as an Effective Medium,”  J. Colloid Interface Sci., 313, 328-337 (2007).

  24. S.A. Allison, H. Pei, Y. Xin, “Modeling the Free Solution and Gel Electrophoresis of Biopolymers:  The Bead Array-Effective Medium Model,” Biopolymers, 87, 102-114  (2007).

  25. H. Pei, Y. Xin, S.A. Allison, “Using Electrophoretic Mobility and Bead Modeling to Characterize the Charge and Secondary Structure of Peptides,”  Journal of Separation Science, 31, 555-564 (2008).

  26. S.A. Allison, H. Pei, B. Haynes, Y. Xin, L. Law, J. Labrum, D. Augustin, “Translational Diffusion of Macromolecules and Nanoparticles Modeled as Non-Overlapping Bead Arrays in an Effective Medium,”  Journal of Physical Chemistry B, 112, 5858-5866 (2008).

  27. S.A. Allison, H. Pei, “The Bead Array-Effective Medium Model for the Electrophoresis of Macromolecules and Nanoparticle Arrays,”  in “Nanoparticles:  New Research Directions”, NOVA publishers, (in press, 12/07).

  28. H. Pei, S.A. Allison, B. Haynes, D. Augustin, “Brownian Dynamics Simulation of the  Diffusion of Rods and Wormlike Chains in a Gel Modeled as a Cubic Lattice:  Application to DNA,”  Journal of Physical Chemistry B, (submitted, 2008).

 

INVITED SEMINARS or TALKS (since 2000)

“Boundary Element Modeling of Biomolecular Transport”, University of Glasgow, Glasgow, Scotland, UK, 9/00

“Transport Properties of Colloids and Macroions Using Detailed Models,”  University of Goteborg, Goteborg, Sweden, 4/02

“Electrokinetic Modeling of Protein Charge Ladders,”  Department of Chemistry and Biochemistry, San Francisco State University, San Francisco, CA, 9/04

“Modeling the Electrophoretic Mobility of Peptides and Proteins in Aqueous Solution,” Southeast Theoretical Chemistry Association (SETCA), Annual Meeting, Emory University, Atlanta, GA., 5/19-20/06.

 

PRESENTATIONS AT PROFESSIONAL MEETINGS (since 2000)

 “Free Solution Electrophoresis and Transport Studies of RNAase,” S. Mazur, J. Kingsbury, T. Laue, and S. Allison, poster presentation at Biophysical Society Meeting, New Orleans, LA, 2/00

“Free Solution Electrophoresis of Lysozyme Charge Mutants,” J.A. Durant, T.M. Laue, T.P. Moody, and S.A. Allison, poster presentation at Biophysical Society Meeting, Boston, MA, 2/01

“Modeling the Free Solution Electrophoresis of T4Lysozyme and Mutants,” C. Chen, S.A. Allison, J. Durant, and T. Laue, poster presentation at Biophysical Society Meeting, San Francisco, CA, 2/02

“Protein Charge and Membrane Confined Electrophoresis (MCE),” J. Durant, T. Moody, T. Laue, S.A. Allison, poster presentation at Biophysical Society Meeting, San Francisco, CA, 2/02

“How Important are Electroviscous Effects in the Characterization of Silica Sols,” S. Wall, M. Rasmusson, and S.A. Allison,  poster presentation at International Symposium on Electrokinetic Phenomena, Cracow, Poland, 8/02

“Modeling Studies on the Transport Properties of Pancreatic Lipase,” F.D. Burkes and S.A. Allison, poster presentation for McNair Underdgraduate Summer Research Program at Georgia State University, Atlanta, Ga., 8/03

“Primary Electroviscous Effects in Silica Sols,”  M. Rasmusson, S. Wall, S.A. Allison, presentation at 11th International Conference on Surface and Colloid Science, Iguassu Falls, Parana, Brazil, 9/03

“Determination of Protein Charge and Folding Using Protein Charge Ladders and Capillary Zone Electrophoresis,” H. Mitchell, N. Raje, E. Breyer, and S. Allison, poster presentation at ABRCMS, San Diego, CA, 10/03

“Determination of Protein Charge and Folding Using Protein Charge Ladders and Capillary Zone Electrophoresis,” H. Mitchell, N. Raje, E. Breyer, and S. Allison, poster presentation at SERMACS, Atlanta, GA, 11/03

“Modeling the Electrophoretic Mobility of Proteins and Peptides,” H. Mitchell, S. Allison, Y. Xin, and H. Cameron, poster presentation for McNair Underdgraduate Summer Research Program at Georgia State University, Atlanta, Ga., 8/05

“Modeling the Electrophoresis of Peptides,” Y. Xin, J. Cobb, N. Ho, and S. Allison, poster presentation for South East Collaborative Alliance Biocomputing Center, SECABC, Fall Workshop on Biocomputing, Georgia State University, Atlanta, Ga., 10/27/05.

“Electrokinetic Gel Layer Model of a Spherical, Highly Charged Colloid Particle,” Y. Xin and S. Allison, poster presentation at the Southeastern Theoretical Chemistry Association (SETCA), Annual Meeting, Emory Universty, Atlanta GA, 5/19-20/06.

“Modeling the Electrophoretic Mobility of DNA and Nucleotides,” H. Mitchell, S. Allison, S. Karobi, and R. Hess, poster presentation for McNair Underdgraduate Summer Research Program at Georgia State University, Atlanta, Ga., 8/06.

 

SIGNIFICANT INSTITUTIONAL SERVICE (since 2000)

Departmental Curriculum Committee, 1994-present

Departmental Executive Committee, 1999-2002

Departmental Bioinformatics Advisor, 2002-present

College Promotion & Tenure Committee, 2000-2003

College of Arts and Sciences Executive Committee, 2006-present

 

RECENT RESEARCH GRANTS (since 2000)

 NSF Proposal (approved), amount: $180,000, grant period 9/1/98-8/31/03, title: "Transport of Polyions in Electric Fields"

GSU QIF Proposal (approved), amount, $34,681, grant period 2000, title: "Biomolecular Size Analyses Using Photon Correlation Spectroscopy," (with E.D. Breyer, T.L. Netzel, and W.D. Wilson)

NSF Proposal (submitted, but not funded), amount: $480,000, grant period 9/05-8/07, title: “Reaction Engineering of Nanostructured Assemblies,” (with J. Carbeck (PI), and S. Shvartsman – (both Carbeck and Shvartsman are faculty members in the Dept. of Chemical Engineering at Princeton